Core--Confocal Imaging- Shared Resource

核心--共焦成像-共享资源

基本信息

批准号：
7038845
负责人：
KAMIAR MOIN
金额：
$ 7.22万
依托单位：
WAYNE STATE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2004
资助国家：
美国
起止时间：
2004-12-01 至 2009-11-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7038845
关键词：
bioimaging /biomedical imaging biomedical facility confocal scanning microscopy fluorescence microscopy immunocytochemistry in situ hybridization intracellular transport light microscopy molecular /cellular imaging neoplasm /cancer protein transport

项目摘要

The purpose of the Confocal Core is to enhance the peer-reviewed funded research activities of Karmanos Cancer Institute members whose research requires confocal microscopy and related techniques. Capabilities and services include: Confocal microscopy, high resolution conventional light microscopy, in vivo small animal imaging, in situ hybridization, cytochemistry, histochemistry, immunocytochemistry, immunohistochemistry, intracellular trafficking studies, multiple labeling studies (up to 3, soon to expand to 7, different parameters can be studied at the same time) and ratiometric analyses (e.g., intracellular pH and ion measurement studies), as well as three dimensional image reconstruction and quantitative measurements. The confocal imaging system consists of a Zeiss LSM 310 confocal microscope equipped with three lasers, a Meridian InsightPlus confocal microscope equipped with one laser and the newly acquired Zeiss LSM 510 META confocal microscope with 4 lasers and multiphoton imaging capability. The facility also provides conventional fluorescent microscopy through a Zeiss Axiophot Triple-Camera Photomicroscope and the newly acquired Zeiss live cell imaging microscope with the state of the art Apotome module capable of four dimensional imaging (3D in time). In vivo small animal optical imaging is a newly acquired technology made available to the core through the purchase of the Kodak IS2000MM small animal imager. The analytical systems of the Confocal Facility include a SiliconGraphics Octane workstation, 4 Macintosh G4 workstations as well as a Dell Precision 650 workstation with various analytical software and photographic quality printers.

共焦核心的目的是加强卡马诺斯癌症研究所成员的同行评审资助的研究活动，这些成员的研究需要共焦显微镜和相关技术。能力和服务包括：共焦显微镜、高分辨率常规光学显微镜、小动物体内成像、原位杂交、细胞化学、组织化学、免疫细胞化学、免疫组织化学、细胞内运输研究、多重标记研究（最多 3 个，很快将扩展到 7 个，可以同时研究不同的参数）和比率分析（例如，细胞内 pH 和离子测量研究），以及三维图像重建和定量测量。共焦成像系统由配备三个激光器的 Zeiss LSM 310 共焦显微镜组成，配备一个激光器的 Meridian InsightPlus 共焦显微镜和新购买的配备 4 个激光器和多光子成像功能的 Zeiss LSM 510 META 共焦显微镜。该设施还通过蔡司 Axiophot 三摄像头光学显微镜和新购置的蔡司活细胞成像显微镜以及能够进行四维成像（3D 时间）的最先进的 Apotome 模块提供传统荧光显微镜。体内小动物光学成像是通过购买柯达IS2000MM小动物成像仪而获得的一项新获得的核心技术。 Confocal 设施的分析系统包括一个 SiliconGraphics Octane 工作站、4 个 Macintosh G4 工作站以及配有各种分析软件和照相质量打印机的 Dell Precision 650 工作站。