ShEEP request for a Zeiss LSM 880 with Airyscan confocal system

ShEEP 请求配备 Airyscan 共焦系统的 Zeiss LSM 880

• 批准号: 9573958
• 负责人: MARK H. TUSZYNSKI
• 金额: --
• 依托单位: VA SAN DIEGO HEALTHCARE SYSTEM
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-01-01 至 2018-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9573958
• 关键词: 15 year old; 18 year old; Aging; Alkalies; Alzheimer' s Disease; Anesthesiology; Biological; Biomedical Research; Blindness; Cardiology; Cells; Clinic; Collaborations; Collection; Color; Complex; Computer software; Computers; Confocal Microscopy; Core Facility; Data; Data Collection; Data Set; Detection; Development; Devices; Diabetes Mellitus; Electron Microscope; Electron Microscopy; Ensure; Equipment; Floor; Fluorescence; Fluorescence Resonance Energy Transfer; Funding; Goals; Head; Health; Heart Diseases; Image; Image Enhancement; Laboratories; Lasers; Light; Light Microscope; Lighting; Maintenance; Manufacturer Name; Medical center; Microscope; Microscopy; Minor; Modeling; Molecular and Cellular Biology; Monitor; Morphologic artifacts; Movement; Nerve Degeneration; Neurology; Noise; Play; Positioning Attribute; Productivity; Program Evaluation; Psychiatry; Request for Applications; Research; Research Personnel; Research Project Grants; Resolution; Resource Sharing; Role; Running; Sampling; Scanning; Signal Transduction; Speed; Spinal cord injury; Support System; System; Techniques; Technology; Time; Traumatic Brain Injury; Veronica; Veterans; Visible Radiation; aging gene; base; cost; design; detector; discount; experimental study; flexibility; gallium arsenide; gene therapy; imaging capabilities; imaging system; improved; innovation; instrument; instrumentation; light microscopy; microscopic imaging; optical spectra; photomultiplier; programs; quantitative imaging; quasar; regenerative; relating to nervous system; repaired; stem cell biology; tool

With this application we request funds from the ORD Shared Equipment Evaluation Program (ShEEP) to purchase a new super resolution confocal microscope system as a shared resource to be housed in the Neurology Program laboratories on the 6th floor of the San Diego VA Medical Center. Specifically, we seek to obtain a model LSM 880 with Airyscan manufactured by Carl Zeiss USA, Inc. (Thornwood, NY). The rationale for this purchase is threefold. First, Current confocal microscope systems available through the San Diego VAMC Microimaging Core Facility (Zeiss LSM 510) and the Center for Neural Repair (Olympus FV1000) are both over 15 years old, are well over their operational lifetimes and as of September 2016 are obsolete and no longer supported by the manufacturers. Going forward, parts and maintenance will no longer be available and these microscopes, which are critical to the research goals of many VA investigators, will become unusable. Second, we are able to take advantage of a time-limited 20% trade-in offer from Zeiss for the purchase of the LSM 880 with Airyscan system. Third, the LSM 880 with Airyscan has many advanced features that place it at the cutting edge of confocal microscopy, including features that are not available from any other manufacturer. Therefore, the acquisition of an LSM 880 with Airyscan confocal microscopy system will dramatically enhance the imaging capabilities available to many VA investigators and support the most demanding research goals. This will certainly stimulate the development of new research projects and new collaborations among investigators at the San Diego VAMC, which has a long-standing record of excellence in research to benefit Veterans health. The research portfolio of the major and minor users for this instrument is very diverse and includes projects studying regenerative approaches to spinal cord injury and neural repair as well as studies investigating the cellular and molecular biology of ageing, gene therapy for Alzheimer's disease as well as for cardiac disease, traumatic brain injury, psychiatry, cardiology, anesthesiology, neurodegeneration, stem cell biology, diabetes and blindness. Access to the most advanced confocal microscopy tools and techniques is critical to all of these studies, as they all involve the collection of large amounts of quantitative image information across a large spatial, temporal and spectral dynamic range from a diverse set of living and fixed biological samples with the highest resolution and speed possible. Specifically, research groups and projects for which the LSM 880 with Airyscan will be critical are: Dr Mark Tuszynski, Dr John Brock, Dr Paul Lu, Dr Brian Head, Dr Robert Rissman, Dr Hemal Patel, Dr Thomas Hnasko, Dr Allen Ryan, Dr Steven Wagner, Dr Dhakshin Ramanathan and Dr Veronica Shubayev, Dr Kirk Hammond and Dr Ephron Rosenszweig. Thus, acquisition of an LSM 880 with Airyscan will not merely maintain confocal microscopy at the San Diego VA Medical Center. Rather, this cutting edge technology will help to ensure that we remain leaders in our respective fields at the forefront of research to improve Veteran's health.

通过此申请，我们向 ORD 共享设备评估计划 (ShEEP) 请求资金 购买新的超分辨率共焦显微镜系统作为共享资源，安装在 位于圣地亚哥退伍军人医疗中心六楼的神经病学项目实验室。具体来说， 我们寻求获得由 Carl Zeiss USA, Inc. 制造的配有 Airyscan 的型号 LSM 880。 （纽约州索恩伍德）。此次购买的理由有三个。一、现有共焦显微镜 系统可通过圣地亚哥 VAMC 显微成像核心设施 (Zeiss LSM 510) 和 神经修复中心 (Olympus FV1000) 均已超过 15 年历史，远超其运营水平 生命周期和截至 2016 年 9 月已过时，制造商不再支持。去 展望未来，零件和维护将不再可用，而这些显微镜对于 许多 VA 研究人员的研究目标将变得无法使用。其次，我们能够采取 购买 LSM 880 可享受 Zeiss 限时 20% 以旧换新优惠 艾里扫描系统。第三，配备 Airyscan 的 LSM 880 具有许多先进功能，使其处于领先地位。 共焦显微镜的尖端技术，包括其他任何显微镜所不具备的功能 制造商。因此，购买配备 Airyscan 共焦显微镜系统的 LSM 880 将 极大地增强了许多 VA 研究人员可用的成像能力，并支持大多数 苛刻的研究目标。这肯定会刺激新研究项目的发展 圣地亚哥 VAMC 的调查人员之间开展了新的合作，该中心拥有长期记录 卓越的研究有益于退伍军人的健康。 该工具的主要和次要用户的研究组合非常多样化，包括 研究脊髓损伤和神经修复再生方法的项目以及研究 研究衰老的细胞和分子生物学以及阿尔茨海默病的基因治疗 至于心脏病、脑外伤、精神病学、心脏病学、麻醉学、 神经退行性疾病、干细胞生物学、糖尿病和失明。获得最先进的共聚焦 显微镜工具和技术对于所有这些研究都至关重要，因为它们都涉及收集 跨越大空间、时间和光谱动态的大量定量图像信息 范围包括具有最高分辨率和速度的各种活体和固定生物样本 可能的。具体来说，LSM 880 与 Airyscan 对其至关重要的研究小组和项目 是： Mark Tuszynski 博士、John Brock 博士、Paul Lu 博士、Brian Head 博士、Robert Rissman 博士、Hemal Patel 博士、 Thomas Hnasko、Allen Ryan 博士、Steven Wagner 博士、Dhakshin Ramanathan 博士和 Veronica 博士 舒巴耶夫、柯克·哈蒙德博士和埃夫隆·罗森茨威格博士。 因此，购买带有 Airyscan 的 LSM 880 将不仅仅在 San 迭戈退伍军人医疗中心。相反，这种尖端技术将有助于确保我们保持 我们各自领域的领导者处于改善退伍军人健康研究的前沿。