The VA Gordon Mansfield SCI Consortium

VA Gordon Mansfield SCI 联盟

• 批准号: 9468259
• 负责人: MARK H. TUSZYNSKI
• 金额: --
• 依托单位: VA SAN DIEGO HEALTHCARE SYSTEM
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-07-01 至 2020-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9468259
• 关键词: Adult; Adverse effects; Anatomy; Animal Model; Autologous; Axon; Brain; California; Cell Therapy; Cells; Chronic; Clinical Trials; Collaborations; Computer Analysis; Contusions; Data; Development; ES Cell Line; Fibrin; Fibroblasts; Forelimb; Funding; Genetic; Graft Survival; Growth Factor; Human; Implant; In Vitro; Injury; Laboratory Research; Lead; Lesion; Macaca mulatta; Medical center; Methods; MicroRNAs; Modeling; Monitor; Monkeys; Natural regeneration; Neuronal Plasticity; Neurons; Nuclear Pore Complex; Pilot Projects; Primates; RNA; Rattus; Recovery of Function; Rehabilitation therapy; Research; Research Personnel; Robot; Rodent; Safety; San Francisco; Scientist; Site; Spastic; Spinal Cord; Spinal Cord Contusions; Spinal cord injury; Stem cells; System; Technology Transfer; Testing; Thrombin; Time; Toxic effect; Training; Translating; Translations; United States; Veterans; Work; chronic pain; clinical practice; deep sequencing; design; experimental study; functional outcomes; in vivo; induced pluripotent stem cell; injured; innovation; model development; nerve stem cell; neural circuit; neural graft; nonhuman primate; pre-clinical; pre-clinical trial; public health relevance; rehabilitation paradigm; rehabilitation strategy; relating to nervous system; repaired; robot assistance; spasticity; stem; stem cell biology; success; therapy development; transdifferentiation; tumor

 DESCRIPTION: The project aims to develop a stem cell-based therapy to repair the chronically injured spinal cord and to maximize functional recovery. Success development of this work can lead to translation of pre-clinical observations from animal models into clinical practice. Moreover, we aim to develop innovative rehabilitation strategies to promote meaningful recovery of function, and objectively determine whether a combination of stem cell-based therapies with rehabilitation will optimize functional outcomes. We will focus on multipotent neural progenitor cells derived from approved embryonic stem cell lines, and on multipotent neural stem /progenitor cells directly transdifferentiated from adult fibroblasts (a form of Induced Pluripotent Stem Cell, IPSC). The work will use both transection and contusion models of spinal cord injury in rodent and non-human primate species. Moreover, we propose replication studies by an investigator that is independent of the consortium of scientists in this project. This is an intensely collaborative effort among leading laboratories researching spinal cord injury located on the west coast of the United States, including the Tuszynski group at the San Diego VA Medical Center and UCSD; the Beattie/Bresnahan group at the San Francisco VA Medical Center and UCSF; the Edgerton group at UCLA; the Havton and Rinkensmeyer groups at UC Irvine; and the California Regional Primate Research Center located at UC Davis. Proposed experiments are collectively designed and performed by this collaboration, an unprecedented effort in spinal cord injury research to move the field forward through close collaboration and interaction on jointly performed experiments. Throughout, this project will benefit from a free exchange of information among the collaborators.

 描述： 该项目旨在开发一种基于干细胞的疗法来修复慢性损伤的脊髓并最大限度地恢复功能。这项工作的成功开发可以将动物模型的临床前观察结果转化为临床实践。制定创新的康复策略以促进有意义的功能恢复，并客观地确定基于干细胞的疗法与康复的结合是否会优化功能结果。我们将重点关注源自批准的胚胎干细胞系的多能神经祖细胞和多能神经祖细胞。干细胞/祖细胞直接从成体成纤维细胞（诱导多能干细胞的一种形式，IPSC）转分化。这项工作将使用啮齿动物和非人类灵长类动物的脊髓损伤模型。此外，我们建议由一名研究人员进行复制研究。该项目独立于科学家联盟，是位于美国西海岸研究脊髓损伤的领先实验室之间的密切合作，其中包括位于美国西海岸的 Tuszynski 小组。圣地亚哥退伍军人管理局医疗中心和加州大学圣地亚哥分校；旧金山退伍军人管理局医疗中心和加州大学旧金山分校的 Beattie/Bresnahan 小组；加州大学洛杉矶分校的 Edgerton 小组；加州大学欧文分校的 Havton 和 Rinkensmeyer 小组以及加州大学戴维斯分校的加州地区灵长类动物研究中心；拟议的实验是由这次合作共同设计和进行的，这是脊髓损伤研究领域前所未有的努力，通过联合进行的实验的密切合作和互动推动该领域的发展。合作者。