X-RAY STRUCTURAL STUDIES OF PROTEINS

蛋白质的 X 射线结构研究

• 批准号: 6489917
• 负责人: BRIAN W MATTHEWS
• 金额: $ 24.49万
• 依托单位: UNIVERSITY OF OREGON
• 依托单位国家: 美国
• 财政年份: 1979
• 资助国家: 美国
• 起止时间: 1979-01-01 至 2002-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6489917
• 关键词: DNA; DNA binding protein; DNA repair; DNA replication; RNA binding protein; X ray crystallography; active sites; adenosinetriphosphatase; bioimaging /biomedical imaging; crystallization; developmental genetics; enzyme mechanism; enzyme structure; genetic recombination; intermolecular interaction; phosphodiesterase I; protein engineering; protein structure function; site directed mutagenesis; structural biology; transcription factor; transcription termination

A major objective of this application is to obtain a better understanding of the interactions between DNA and proteins and of related biological activities including the regulation of gene expression and the mechanisms of DNA replication, transcription and recombination. Toward this end crystallographic, biochemical and mutagenic studies will be pursued of a number of DNA- and RNA-interacting proteins including the exonuclease from phage lambda, the Skn-1 developmental regulatory protein from C. elegans, the "N" antitermination protein and DNA replication proteins from phage T4 as well as various engineered and other variants of lambda Cro protein. Studies will also be continued of E. coli beta-galactosidase. The way in which proteins interact with DNA is fundamental to the correct regulation and growth of every living cell. Many human diseases have their origins in a breakdown in these basic control processes. Studies of the sort described here provide fundamental insights into the way in which proteins recognize their target sites on DNA and exert their control functions. There is every reason to expect that understandings derived from such systems in simple organisms will be relevant to human health. Knowledge of the three-dimensional structure of E. coli beta-galactosidase is expected to lead to improvements in a number of the assays that are based on the unique properties of the enzyme.

该应用程序的一个主要目标是获得更好的 了解 DNA 和蛋白质之间的相互作用 相关的生物活性，包括基因的调控 DNA复制、转录和表达的机制 重组。 为此，晶体学、生物化学和诱变研究将 可以追踪许多 DNA 和 RNA 相互作用的蛋白质，包括 噬菌体 lambda 的核酸外切酶、Skn-1 发育调节酶 来自线虫的蛋白质，“N”抗终止蛋白和 DNA 来自噬菌体 T4 的复制蛋白以及各种工程化和 lambda Cro 蛋白的其他变体。 也将继续研究 大肠杆菌β-半乳糖苷酶。 蛋白质与 DNA 相互作用的方式是 每个活细胞的正确调节和生长。 人类多种疾病 其根源在于这些基本控制过程的崩溃。 这里描述的这类研究提供了对以下问题的基本见解： 蛋白质识别 DNA 上的目标位点并发挥作用的方式 他们的控制功能。有充分理由期待这一点 从简单生物体中的此类系统中得出的理解将是 与人类健康相关。 三维结构知识 大肠杆菌β-半乳糖苷酶的使用有望改善 基于独特性质的测定数量 酶。