STRUCTURAL STUDIES OF NUCLEIC ACID-BINDING PROTEINS

核酸结合蛋白的结构研究

• 批准号: 7370357
• 负责人: BRIAN W MATTHEWS
• 金额: $ 0.02万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-03-01 至 2007-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7370357
• 关键词: STRUCTURAL; STUDIES; NUCLEIC; ACID; BINDING

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Recognition and binding of nucleic acids by proteins plays a fundamental role in cellular biochemistry. We are interested in studying the structural basis for specific and non-specific protein-nucleic acid interactions. We are investigating non-specific DNA-binding by elucidating the mechanisms of processivity of two distinct DNA exonucleases through structural studies. Specific protein-DNA interactions will be investigated using a Drosophila developmental protein in complex with DNA. In order to gain better insight into the structural basis of protein-RNA interactions, we are looking at a bacterial DEAD-box RNA helicase. These structural studies will provide a better understanding of the structural characteristics that govern protein-nucleic acid interactions.

该子项目是利用 NIH/NCRR 资助的中心拨款提供的资源的众多研究子项目之一。子项目和研究者 (PI) 可能已从另一个 NIH 来源获得主要资金，因此可以在其他 CRISP 条目中出现。列出的机构是中心的机构，不一定是研究者的机构。蛋白质对核酸的识别和结合在细胞生物化学中起着基本作用。我们有兴趣研究特定和非特定蛋白质-核酸相互作用的结构基础。我们正在通过结构研究阐明两种不同 DNA 核酸外切酶的持续合成机制，从而研究非特异性 DNA 结合。将使用与 DNA 复合的果蝇发育蛋白来研究特定的蛋白质-DNA 相互作用。为了更好地了解蛋白质-RNA 相互作用的结构基础，我们正在研究细菌 DEAD-box RNA 解旋酶。这些结构研究将有助于更好地理解控制蛋白质-核酸相互作用的结构特征。