REAL-TIME MICROSCOPIC IMAGING OF FAST MEMBRANE POTENTIA

快速膜电位的实时显微成像

• 批准号: 6530140
• 负责人: Vladislav V. Yakovlev
• 金额: $ 10.26万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-30 至 2005-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6530140
• 关键词: Xenopus; action potentials; axon; bioengineering /biomedical engineering; computer data analysis; confocal scanning microscopy; developmental neurobiology; membrane potentials; neuromuscular junction; neurophysiology; noninvasive diagnosis; synaptogenesis; time resolved data

DESCRIPTION (provided by applicant): This proposal takes advantage of advances in laser technology that make it possible to detect electrical activity in unstained living tissue in a noninvasive manner. The first aim is to build a laser microscope that can detect SH signals generated by focused laser radiation at wavelengths that will minimize the possibility of tissue damage. The optics and data acquisition will be engineered for optimal use in a noninvasive manner and to maximize user-friendliness. The second aim is to evaluate and test the sensitivity of the microscope including its spatial and temporal resolution in detecting action potentials in a classical neurophysiological preparation. The third aim is to use the SH microscope for pilot tests on the functional development of synapses at the developing neuromuscular junction of vertebrates. These studies could provide information about the mechanisms of synapse formation that are not currently possible with other techniques. In addition, the ability to measure activity at the neuromuscular junction in a noninvasive way would have value in a number of clinical applications. Diseases like Myasthenia Gravis, the Muscular Dystrophies, ALS, and Guillain-Barr syndrome affect the activity of nerve and/or muscle cells. Optical microscopy could lead to new diagnostic methods and a better understanding of the mechanisms of disease progression.

描述（由申请人提供）：该提案利用了激光技术的进步，使检测电气成为可能 以非侵入性方式在未染色的活组织中进行活性。 第一个目标是 建立一个激光显微镜，可以检测聚焦产生的SH信号 激光辐射的波长可最大限度地减少组织损伤的可能性 损害。 光学和数据采集将被设计为最佳使用 以非侵入性方式并最大限度地提高用户友好性。 第二个目标是 评估和测试显微镜的灵敏度，包括其空间和 经典动作电位检测中的时间分辨率 神经生理学准备。 第三个目标是使用 SH 显微镜 发育中突触功能发育的试点测试 脊椎动物的神经肌肉接头。 这些研究可以提供 有关突触形成机制的信息目前还没有 可以使用其他技术。 此外，测量活动的能力 以非侵入性方式在神经肌肉接头处进行治疗将具有价值 临床应用数量。 重症肌无力等疾病 肌营养不良症、ALS 和格林-巴尔综合征会影响 神经和/或肌肉细胞。 光学显微镜可能带来新的诊断方法 方法并更好地了解疾病进展的机制。

项目成果

How to measure chi(3) of a nanoparticle.

如何测量纳米颗粒的 chi(3)。

• 发表时间: 2006-05-15
• 影响因子: 3.6
• 作者: Shcheslavskiy, Vladislav I;Saltiel, Solomon M;Faustov, Alexey R;Petrov, Georgi I;Yakovlev, Vladislav V
• 通讯作者: Yakovlev, Vladislav V

Effect of photonic crystal structure on the nonlinear optical anisotropy of birefringent porous silicon.

光子晶体结构对双折射多孔硅非线性光学各向异性的影响。

• 发表时间: 2006-11-01
• 影响因子: 3.6
• 作者: Petrov, Georgi I;Shcheslavskiy, Vladislav I;Yakovlev, Vladislav V;Golovan, Leonid A;Krutkova, Elena Yu;Fedotov, Andrei B;Zheltikov, Alexei M;Timoshenko, Victor Yu;Kashkarov, Pavel K;Stepovich, Ekaterina M
• 通讯作者: Stepovich, Ekaterina M