NONLINEAR OPTICAL CONFOCAL MICROSCOPE

非线性光学共焦显微镜

• 批准号: 6051640
• 负责人: WILLIAM J BETZ
• 金额: $ 50万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-04-01 至 2002-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6051640
• 关键词: bioimaging /biomedical imaging; biomedical equipment purchase; confocal scanning microscopy

We request funds to purchase a Zeiss LSM 510 NLO confocal microscope, augmented by a Coherent MiraX-ZFC laser system for multi- photon microscopy. Seven Major Users and eleven other users, well funded by NIH (26 separate research grants), describe twenty research projects that span a broad range of biological questions and will collectively utilize all of the capabilities of the instrument. The applicants represent seven different departments in the School of Medicine. The Dean has made a commitment of $200,000 for support of the facility, and other faculty have made additional commitments of funds, equipment, and space. Dr. Winfried Denk (Bell Labs) will serve as External Advisor. He and Dr. Graham Ellis-Davies (Philadelphia) will collaborate with Major Users on two research projects. A new graduate 6ourse, "Optical Measurement of Cellular Function in Live Cells" will be offered as part of an expanding optical microscopy facility. Major Users have had extensive experience with various imaging techniques, and have acquired sound preliminary data that illustrate both the need for this equipment and the feasibility of the proposed experiments. A comprehensive inventory of existing equipment and careful scrutiny of proposals make clear that none of the projects can be accomplished with current equipment at the Health Science Center or any other campus in reasonable proximity. A stable and time-tested management plan that has emerged over the past ten years of continuous administration of shared optical facilities by the same personnel will insure that the instrument will be well maintained, that it will become self-supporting, and that it will be used intensively by well- trained scientists drawn both from the User Group and, over time, from the larger population of scientists in the region.

我们请求资金购买 Zeiss LSM 510 NLO 共焦显微镜，并配备用于多光子显微镜的 Coherent MiraX-ZFC 激光系统。七位主要用户和十一位其他用户在 NIH 的大力资助下（26 项单独的研究资助）描述了 20 个研究项目，这些项目涵盖了广泛的生物学问题，并将共同利用该仪器的所有功能。申请人代表医学院的七个不同院系。院长承诺提供 20 万美元用于支持该设施，其他教员也承诺提供资金、设备和空间。 Winfried Denk 博士（贝尔实验室）将担任外部顾问。他和 Graham Ellis-Davies 博士（费城）将与主要用户合作开展两个研究项目。新毕业的 6 门课程“活细胞中细胞功能的光学测量”将作为扩大的光学显微镜设施的一部分提供。主要用户在各种成像技术方面拥有丰富的经验，并获得了合理的初步数据，这些数据说明了对该设备的需求以及所提出的实验的可行性。对现有设备的全面清查和对提案的仔细审查表明，使用健康科学中心或任何其他合理邻近校园的现有设备都无法完成任何项目。在过去十年中，由同一人员对共享光学设施进行连续管理，形成了一个稳定且经过时间考验的管理计划，将确保该仪器得到良好维护，使其能够自给自足，并且能够由来自用户组以及随着时间的推移，来自该地区更多科学家群体的训练有素的科学家集中使用。

项目成果

Dense core vesicle dynamics in Caenorhabditis elegans neurons and the role of kinesin UNC-104.

秀丽隐杆线虫神经元中的致密核心囊泡动力学和驱动蛋白 UNC-104 的作用。

• 发表时间: 2004-07
• 作者: Zahn, Tobias R;Angleson, Joseph K;MacMorris, Margaret A;Domke, Erin;Hutton, John F;Schwartz, Cindi;Hutton, John C
• 通讯作者: Hutton, John C