Two-dimensional MR Spectroscopic Characterization of HE

HE 的二维磁共振波谱表征

• 批准号: 6580234
• 负责人: MICHAEL Albert THOMAS
• 金额: $ 31.72万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-12-04 至 2006-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6580234
• 关键词: aspartate; basal ganglia; bioimaging /biomedical imaging; brain imaging /visualization /scanning; brain metabolism; cingulate gyrus; clinical research; cognition; gamma aminobutyrate; glutamates; glutamine; hepatic coma /encephalopathy; human subject; magnetic resonance imaging; method development; neuropsychological tests; neuropsychology; nuclear magnetic resonance spectroscopy; patient oriented research

DESCRIPTION (provided by applicant): The major goals of this application are to evaluate a localized two-dimensional (2D) MR spectroscopic technique (MRS) and to detect cerebral metabolites unequivocally in hepatic encephalopathy (HE). Detection of early changes is very critical in the diagnosis of HE. Localized MR Spectroscopy is a major biochemical tool in characterizing noninvasively several metabolites in human tissues. Excellent one-dimensional (1D) MR spectra of HE and other pathologies have been reported with promising results. However, spectral overlap of various metabolites has been a major concern with the conventional 1D MRS. Spectral analysis of certain metabolites, namely glutamate, glutamine, GABA, myo-inositol, aspartate, glutathione, and the aspartyl groups of NAA and NAAG, has been complicated by severe overlap at 1.5T. 2D MRS seems well suited to address this problem of overlap. It has revolutionized the applications of NMR in biological macromolecules. Previous attempts by other researchers to implement the localized versions of the two-dimensional MR techniques mainly used phantom solutions, although some results were reported from animal brains using a non-localized 2D technique. In addition to the major metabolites successfully assigned and analyzed so far with 1D MRS, more metabolites are yet to be detected and their respective roles in different pathologiesare yet to be explored using 2D MRS. A major hypothesis to be tested in this work is whether the changes of glutamine/glutamate, myo-inositol, aspartate, GABA and other cerebral metabolites can be detected using the localized 2D COSY more unambiguously in HE than the conventional 1D MRS. Three specific goals of this project will be: 1) to acquire the single-voxel based 2D COSY spectra in the anterior cingulate and the basal ganglia regions of HE patients; 2) to record the global pallidal Mill changes in HE patients; 3) to perform the neuropsychiatric and neurocognitive assessment in HE patients, and to correlate the MR findings with the neuropsycho-logical, blood ammonia and liver function test results using multivariate regression analysis. AGE 1.5T MRI scanner with a bilateral surface "receive" coil (MRI Devices Corp.) in combination with a body rf "transmit" coil will be used to achieve improved signal detection. Minimal spectral overlap leading to less ambiguous assignment and better quantitation of glutamate/glutamine, GABA, myo-inositol, threonine, glucose, taurine and glutathione, will be a major outcome of this work.

描述（由申请人提供）：本申请的主要目标是评估局部二维（2D）MR光谱技术（MRS），并在肝性脑病（HE）中明确检测脑代谢物。检测早期变化对于HE诊断至关重要。局部MR光谱法是表征人类组织中几种代谢物的主要生化工具。据报道，出色的HE和其他病理的优秀一维（1D）MR光谱有令人鼓舞的结果。但是，各种代谢产物的光谱重叠一直是常规1D MRS的主要问题。某些代谢产物，即谷氨酸，谷氨酰胺，GABA，肌醇，天冬氨酸，谷胱甘肽和NAA和NAAG的Aspartyl群的光谱分析已因1.5T的严重重叠而变得复杂。 2d MRS似乎非常适合解决这个重叠问题。它彻底改变了NMR在生物大分子中的应用。其他研究人员先前尝试实施二维MR技术的局部版本，主要使用幻影溶液，尽管使用非关注的2D技术报告了动物大脑的一些结果。除了到目前为止通过一维MRS成功分配和分析的主要代谢产物外，还需要检测到更多代谢物，并且它们在不同的病理学中的各自作用尚未使用2D MRS探索。在这项工作中要检验的一个主要假设是，是否可以使用局部2D舒适的局部化舒适的人比传统的1D MRS更明确地检测到谷氨酰胺/谷氨酸，肌醇，天冬氨酸，GABA和其他脑代谢物的变化。该项目的三个具体目标将是：1）在前扣带回和HE患者的基底神经节中获得基于单素的2D舒适光谱； 2）记录HE患者的全球苍白磨机变化； 3）在患者中进行神经精神和神经认知评估，并使用多变量回归分析将MR发现与神经精神逻辑，血液氨和肝功能测试结果相关。具有双侧表面“接收”线圈（MRI设备公司）的1.5T MRI扫描仪与身体RF“ Transmit”线圈结合使用，以实现改进的信号检测。最小的光谱重叠会导致较小的歧义分配和更好地定量谷氨酸/谷氨酰胺，GABA，Myo-肌醇，苏氨酸，苏氨酸，葡萄糖，牛磺酸和谷胱甘肽，将是这项工作的主要结果。