Two-dimensional MR Spectroscopic Characterization of HE

HE 的二维磁共振波谱表征

基本信息

批准号：
6989743
负责人：
MICHAEL Albert THOMAS
金额：
$ 33.33万
依托单位：
UNIVERSITY OF CALIFORNIA LOS ANGELES
依托单位国家：
美国
项目类别：
财政年份：
2002
资助国家：
美国
起止时间：
2002-12-04 至 2008-11-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6989743
关键词：
Two dimensional MR Spectroscopic Characterization

项目摘要

DESCRIPTION (provided by applicant): The major goals of this application are to evaluate a localized two-dimensional (2D) MR spectroscopic technique (MRS) and to detect cerebral metabolites unequivocally in hepatic encephalopathy (HE). Detection of early changes is very critical in the diagnosis of HE. Localized MR Spectroscopy is a major biochemical tool in characterizing noninvasively several metabolites in human tissues. Excellent one-dimensional (1D) MR spectra of HE and other pathologies have been reported with promising results. However, spectral overlap of various metabolites has been a major concern with the conventional 1D MRS. Spectral analysis of certain metabolites, namely glutamate, glutamine, GABA, myo-inositol, aspartate, glutathione, and the aspartyl groups of NAA and NAAG, has been complicated by severe overlap at 1.5T. 2D MRS seems well suited to address this problem of overlap. It has revolutionized the applications of NMR in biological macromolecules. Previous attempts by other researchers to implement the localized versions of the two-dimensional MR techniques mainly used phantom solutions, although some results were reported from animal brains using a non-localized 2D technique. In addition to the major metabolites successfully assigned and analyzed so far with 1D MRS, more metabolites are yet to be detected and their respective roles in different pathologiesare yet to be explored using 2D MRS. A major hypothesis to be tested in this work is whether the changes of glutamine/glutamate, myo-inositol, aspartate, GABA and other cerebral metabolites can be detected using the localized 2D COSY more unambiguously in HE than the conventional 1D MRS. Three specific goals of this project will be: 1) to acquire the single-voxel based 2D COSY spectra in the anterior cingulate and the basal ganglia regions of HE patients; 2) to record the global pallidal Mill changes in HE patients; 3) to perform the neuropsychiatric and neurocognitive assessment in HE patients, and to correlate the MR findings with the neuropsycho-logical, blood ammonia and liver function test results using multivariate regression analysis. AGE 1.5T MRI scanner with a bilateral surface "receive" coil (MRI Devices Corp.) in combination with a body rf "transmit" coil will be used to achieve improved signal detection. Minimal spectral overlap leading to less ambiguous assignment and better quantitation of glutamate/glutamine, GABA, myo-inositol, threonine, glucose, taurine and glutathione, will be a major outcome of this work.

描述（由申请人提供）：本申请的主要目标是评估局部二维（2D）磁共振波谱技术（MRS）并明确检测肝性脑病（HE）中的脑代谢物。检测早期变化对于 HE 的诊断非常重要。局部磁共振波谱是一种主要的生化工具，可用于非侵入性地表征人体组织中的多种代谢物。已经报道了 HE 和其他病理的优秀一维 (1D) MR 光谱，并取得了有希望的结果。然而，各种代谢物的光谱重叠一直是传统一维 MRS 的主要问题。某些代谢物（即谷氨酸、谷氨酰胺、GABA、肌醇、天冬氨酸、谷胱甘肽以及 NAA 和 NAAG 的天冬氨酰基）的光谱分析因 1.5T 处的严重重叠而变得复杂。二维 MRS 似乎非常适合解决重叠问题。它彻底改变了核磁共振在生物大分子中的应用。其他研究人员之前尝试实现二维 MR 技术的局部版本主要使用幻像解决方案，尽管使用非局部二维技术从动物大脑中报告了一些结果。除了迄今为止使用 1D MRS 成功分配和分析的主要代谢物外，还有更多代谢物有待检测，并且它们在不同病理中各自的作用也有待使用 2D MRS 探索。这项工作要测试的一个主要假设是，在 HE 中使用局部 2D COSY 是否可以比传统 1D MRS 更明确地检测谷氨酰胺/谷氨酸、肌醇、天冬氨酸、GABA 和其他脑代谢物的变化。该项目的三个具体目标是：1）获取 HE 患者前扣带回和基底神经节区域基于单体素的 2D COSY 光谱； 2）记录HE患者的全局苍白球Mill变化； 3) 对 HE 患者进行神经精神和神经认知评估，并使用多元回归分析将 MR 结果与神经心理、血氨和肝功能测试结果相关联。带有双侧表面“接收”线圈（MRI Devices Corp.）的 AGE 1.5T MRI 扫描仪与人体射频“发射”线圈相结合，将用于实现改进的信号检测。这项工作的主要成果将是最小化光谱重叠，从而减少谷氨酸/谷氨酰胺、GABA、肌醇、苏氨酸、葡萄糖、牛磺酸和谷胱甘肽的模糊分配和更好的定量。

项目成果

期刊论文数量（7）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Implementation of multi-echo-based correlated spectroscopic imaging and pilot findings in human brain and calf muscle.

DOI：
10.1002/jmri.22624
发表时间：
2011-08
期刊：
JOURNAL OF MAGNETIC RESONANCE IMAGING
影响因子：
4.4
作者：
Verma, Gaurav;Lipnick, Scott;Ramadan, Saadallah;Nagarajan, Rajakumar;Thomas, M. Albert
通讯作者：
Thomas, M. Albert

Implementation and validation of localized constant-time correlated spectroscopy (LCT-COSY) on a clinical 3T MRI scanner for investigation of muscle metabolism.

在临床 3T MRI 扫描仪上实施和验证局部恒定时间相关光谱 (LCT-COSY)，用于研究肌肉代谢。

DOI：
10.1002/jmri.20990
发表时间：
2007
期刊：
Journal of magnetic resonance imaging : JMRI
影响因子：
0
作者：
Velan,SSendhil;Ramamurthy,Senthil;Ainala,Srilatha;Durst,Christopher;Lemieux,SusanK;Raylman,RaymondR;Spencer,RichardG;Thomas,MAlbert
通讯作者：
Thomas,MAlbert

Magnetic resonance T2-relaxometry and 2D L-correlated spectroscopy in patients with minimal hepatic encephalopathy.

轻微肝性脑病患者的磁共振 T2 弛豫测量和 2D L 相关光谱。

DOI：
10.1002/jmri.21943
发表时间：
2009
期刊：
Journal of magnetic resonance imaging : JMRI
影响因子：
0
作者：
Singhal,Aparna;Nagarajan,Rajakumar;Kumar,Rajesh;Huda,Amir;Gupta,RakeshK;Thomas,MAlbert
通讯作者：
Thomas,MAlbert

Echo planar correlated spectroscopic imaging: implementation and pilot evaluation in human calf in vivo.

DOI：
10.1002/mrm.22499
发表时间：
2010-10
期刊：
MAGNETIC RESONANCE IN MEDICINE
影响因子：
3.3
作者：
Lipnick, Scott;Verma, Gaurav;Ramadan, Saadallah;Furuyama, Jon;Thomas, M. Albert
通讯作者：
Thomas, M. Albert

Role of Magnetic Resonance in Understanding the Pathogenesis of Hepatic Encephalopathy.

磁共振在了解肝性脑病发病机制中的作用。