ABI Prism 7900HT Sequence Detection System

ABI Prism 7900HT 序列检测系统

• 批准号: 6441075
• 负责人: BA-BIE TENG
• 金额: $ 13.06万
• 依托单位: UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-01 至 2003-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6441075
• 关键词: biomedical equipment purchase; high throughput technology; nucleic acid sequence; polymerase chain reaction; single nucleotide polymorphism

We are requesting an ABI Prism 7900HT Sequence Detection System with the automation accessary. This instrument will be housed in the Vector Development Core Laboratory at the Institute of Molecular Medicine. The group of investigators participating in this grant application includes ten researchers with NIH-supported grant projects and three potential young investigators. The investigators are from the Institute of Molecular Medicine and the Medical School from UT- Houston and the Institute of Biosciences and Technology from Texas A&M University. The research projects involved are diverse, ranging from basic biology, cancer research, the identification of target genes in various diseases, gene therapy, and investigating differential gene expression in tissues from human and animals to clinical studies involving the genotyping of susceptibility to Scleroderma and stroke. We intend to use this instrument for the high-throughput real-time quantitative analysis of RNA transcripts and DNA targets in biological samples and for single nucleotide polymorphism genotyping. As indicated in the research project. from each investigator, the availability of the proposed instrument will significantly improve the scope of each one's study. It will allow us to rapidly follow up the exciting discoveries from our currently funded projects. The goal of this multidisciplinary collaborative effort is to develop and share the advanced technology resources needed to carry out the cutting-edge scientific work that will characterize medical research of the future. Most importantly, the established investigators will be able to provide exposure to students and medical fellows to this powerful instrument and to have an environment with new biological tools in which young potential investigators can develop his/her own scientific research. The requested instrument is a high-throughput real-time PCR system that detects and quantifies nucleic acid sequences. An automation accessory with a 96- or 384-well plate capacity facilitates 24-hour unattended operation. The integrated bar code readers simplify sample tracking. The 7900HT system is a remarkably simple instrument to operate. The entire RT-PCR run will be completed in 2 h and the individual can then retrieve his/her results and analyze the data using the software installed in his/her own computer. The ABI Prism Sequence Detection System has been proven to be a stable and easy to operate. The availability of this instrument is essential for improving our current funded projects.

我们要求使用自动化附件的ABI PRISM 7900HT序列检测系统。该仪器将安置在分子医学研究所的矢量发展核心实验室中。参加此赠款申请的研究人员小组包括10位拥有NIH支持的赠款项目和三名潜在的年轻调查人员的研究人员。研究人员来自分子医学研究所和医学院，来自乌特休斯顿和得克萨斯州A＆M大学的生物科学与技术研究所。所涉及的研究项目各不相同，从基本生物学，癌症研究，各种疾病中靶基因的鉴定，基因治疗以及研究从人和动物的组织中的差异基因表达到临床研究，涉及对硬皮病和中风的易感性的基因分型。我们打算将此仪器用于生物样品中的RNA转录本和DNA靶标的高通量实时定量分析以及单核苷酸多态性基因分型。如研究项目所示。从每个研究者那里，提议的仪器的可用性将显着提高每个人研究的范围。它将使我们能够迅速跟进目前资助的项目中令人兴奋的发现。这项多学科合作努力的目的是开发和分享从事未来医学研究的尖端科学工作所需的先进技术资源。最重要的是，知名的研究人员将能够向学生和医疗研究员提供这种强大的工具的接触，并拥有具有新的生物学工具的环境，其中年轻的潜在研究人员可以在其中发展自己的科学研究。请求的仪器是一种高通量实时PCR系统，可检测和量化核酸序列。带有96或384孔板容量的自动化配件有助于24小时无人值守的操作。集成的条形码读取器简化了样本跟踪。 7900HT系统是一种非常简单的操作工具。整个RT-PCR运行将在2小时内完成，然后个人可以检索自己的结果，并使用安装在自己的计算机中的软件分析数据。 ABI PRISM序列检测系统已被证明是稳定且易于操作的。该工具的可用性对于改善我们当前的资金项目至关重要。