Pyrosequencer

焦磷酸测序仪

• 批准号: 6730883
• 负责人: Isaac I Wirgin
• 金额: $ 12.01万
• 依托单位: NEW YORK UNIVERSITY SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2005-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6730883
• 关键词: biomedical equipment purchase; genotype; high throughput technology; nucleic acid sequence; polymerase chain reaction; single nucleotide polymorphism

DESCRIPTION (provided by applicant): Efforts to evaluate the relationship between genetic polymorphisms and susceptibilities to various types of cancers have intensified in recent years in the Department of Environmental Medicine and have resulted in successful collaborative NIH grant applications between investigators in the Epidemiology and Molecular Toxicology/Carcinogenesis Programs in the Department. Additionally, several members of the Department have long NIH funded track records in evaluating the epigenetic effects of DNA methylation at CpG islands in carcinogenesis models. Furthermore, increased efforts in these areas are reflected in Environmental Medicine's successful acquisition of a Superfund Basic Research Program Center Grant entitled "Genetic/Epigenetic Susceptibility to Superfund Chemicals." Other NIH funded projects by Department investigators involve the development of linkage maps with single nucleotide polymorphisms as markers to identify novel genes associated with toxicant induced disease. With this application, we propose to acquire a Pyrosequencer TM to be housed in the shared Molecular Facility Core of Environmental Medicine's longstanding NIEHS Center. This instrument is needed to increase the throughput and accuracy of genotyping for the multiple NIH funded genotyping studies by members of this Department. By increasing sample and loci number, these investigations will be much more sensitive in evaluating the effects of genetic polymorphisms on susceptibility to disease. Similarly, investigators studying the relationships between methylation status and carcinogenesis will be able to expand their analyses to include multiple CpG islands within a short stretch of DNA and will now be able to quantitatively estimate the percentage of hypermethylated nucleotides at individual islands. The NIH grant support obtained by the several research groups needing this instrument shows that they are conducting high-quality science; with the acquisition of the Pyrosequencer they will be able to perform more extensive and detailed investigations that will enable them to stay at the forefront in their research areas.

描述（由申请人提供）：评估遗传多态性和与各种类型癌症之间的关系的努力在近年来在环境医学系中加剧了，并导致了流行病学和分子毒理学研究人员之间的成功合作NIH赠款应用/部门的致癌计划。此外，该部门的几名成员长期以来一直在评估CPG岛上DNA甲基化在致癌模型中的表观遗传作用方面资助了往绩记录。此外，在这些领域的努力增加反映在环境医学成功地获得超级基础研究计划中心的赠款中，名为“遗传/表观遗传学对超级基金化学品的敏感性”。部门调查人员资助的其他NIH资助项目涉及与单核苷酸多态性的连锁图的开发，作为标志物，以识别与毒性诱导疾病相关的新基因。通过此应用，我们建议收购pyrosequencer tm，该TM被安置在环境医学长期存在的NIEHS中心的共享分子设施中。需要该工具来提高该部门成员的多个NIH资助基因分型研究的基因分型的吞吐量和准确性。通过增加样本和基因座数，这些研究将在评估遗传多态性对疾病易感性的影响方面更加敏感。同样，研究甲基化状态与癌变之间关系的研究者将能够扩展其分析，以在短暂的DNA范围内包括多个CpG岛，现在将能够定量估计单个岛屿上高甲基化核苷酸的百分比。需要该工具的几个研究小组获得的NIH赠款支持表明，他们正在进行高质量的科学；通过收购Pyrosequencer，他们将能够进行更广泛和详细的调查，使他们能够保持研究领域的最前沿。