ABI PRISM 7900HT Sequence Detection System

ABI PRISM 7900HT 序列检测系统

• 批准号: 6730868
• 负责人: JEANETTE Christine PAPP
• 金额: $ 10.5万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2005-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6730868
• 关键词: biomedical equipment purchase; high throughput technology; nucleic acid quantitation /detection; nucleic acid sequence; polymerase chain reaction

DESCRIPTION (provided by applicant): With the recent rapid explosion in laboratory instrumentation, genetics has become a technology-driven field. High technology, however, comes with a high price tag. It can prove impossible for any but the largest, most well-funded laboratories to find the resources to move from one cutting-edge technology to the next as their research progresses. To address this problem, the University of California, Los Angeles has made a commitment to supporting and facilitating the research of all its scientists by providing a system of state-of-the-art Core facilities. These Cores are staffed and equipped to allow scientists to concentrate more time on intellectual problem solving, and less on acquiring and learning new technologies. The UCLA Sequencing and Genotyping Core Facility, with its Advisory Committee made up of some of the top geneticists in the world, and its close relationship with a very large user base in the local scientific community, is in an excellent position to identify not only the future direction of genetic research, but also the needs of local scientists. We have identified a strong need for an inexpensive method to generate large numbers of SNP genotypes, and a technology to perform gene expression experiments once the genes have been identified. The Sequencing and Genotyping Core currently offers several technologies for SNP genotyping, and also operates an ABI 7700 Taqman instrument for gene expression experiments. With Taqman technology, both SNP genotyping and gene expression are possible on a single platform. However, the instruments the Core currently maintains are inadequate in cost per reaction and in throughput to keep up with the evolving requirements of research with these two applications. The high-throughput ABI 7900HT Taqman platform would serve as a solution, employing Taqman technology to offer large-scale SNP genotyping, quantitative PCR, and gene expression capabilities all on a single, moderately priced instrument. The Sequencing and Genotyping Core already has extensive experience with this technology on the Core's current ABI 7700, along with the proven infrastructure and expertise to maintain and administer the technology, both upstream in the laboratory and downstream in the data management and analysis arenas. The ABI 7900HT could be rapidly brought on-line, and provide a valuable service to a large existing and expanding client base of NIH-funded scientists at UCLA and in the broader scientific community.

描述（由申请人提供）：随着近年来实验室仪器的快速发展，遗传学已成为一个技术驱动的领域。 然而，高科技伴随着高昂的价格。事实证明，除了最大、资金最充足的实验室之外，随着研究的进展，任何实验室都不可能找到资源从一种尖端技术转向另一种尖端技术。为了解决这个问题，加州大学洛杉矶分校承诺通过提供最先进的核心设施系统来支持和促进所有科学家的研究。这些核心的人员配备和装备使科学家能够将更多时间集中在解决智力问题上，而不是花在获取和学习新技术上。加州大学洛杉矶分校测序和基因分型核心设施的咨询委员会由世界上一些顶尖的遗传学家组成，并且与当地科学界的庞大用户群有着密切的关系，它处于一个非常有利的位置，不仅可以识别未来基因研究的方向，也是当地科学家的需要。我们已经确定迫切需要一种廉价的方法来生成大量 SNP 基因型，以及一种在基因被识别后进行基因表达实验的技术。测序和基因分型核心目前提供多种 SNP 基因分型技术，还运行 ABI 7700 Taqman 仪器进行基因表达实验。借助 Taqman 技术，可以在单一平台上进行 SNP 基因分型和基因表达。然而，核心目前维护的仪器在每次反应成本和通量方面都不足以满足这两种应用不断变化的研究需求。高通量 ABI 7900HT Taqman 平台将作为一种解决方案，利用 Taqman 技术在一台价格适中的单一仪器上提供大规模 SNP 基因分型、定量 PCR 和基因表达功能。 测序和基因分型核心已经在其当前的 ABI 7700 上拥有该技术的丰富经验，以及维护和管理该技术的成熟基础设施和专业知识，无论是在实验室上游还是在数据管理和分析领域的下游。 ABI 7900HT 可以快速上线，并为加州大学洛杉矶分校和更广泛的科学界由 NIH 资助的科学家组成的大量现有和不断扩大的客户群提供有价值的服务。