AB 3730XL, 96 CAP DNA ANALYZER

AB 3730XL，96 CAP DNA 分析仪

• 批准号: 7335130
• 负责人: JEANETTE Christine PAPP
• 金额: $ 36.81万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-15 至 2007-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7335130
• 关键词: DNA; capillary; gait; genotype; model

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): The demand for genotyping is increasing rapidly, fuelled by research into complex traits through large association studies, with large numbers of samples and SNPs. At the same time, the need for microsatellite genotyping and sequencing continues to exist in at least their current levels. Therefore, the capacity for rapid, cheap, high-throughput sequencing and genotyping must increase. The recent rapid explosion in laboratory instrumentation is well able to keep pace with this increasing need. However, this high technology comes with a high price tag. It can prove impossible for any but the largest, most well-funded laboratories to find the resources to move from one cutting-edge technology to the next as their research progresses. To address this problem, the University of California, Los Angeles has made a commitment to supporting and facilitating the research of all its scientists by providing a system of state-of-the-art Core facilities. These Cores are staffed and equipped to allow scientists to concentrate more time on intellectual problem solving, and less on acquiring and learning new technologies. The UCLA Sequencing and Genotyping Core serves the research needs of a large user base in the local scientific community. We have identified a coming need for an inexpensive method to generate increasingly large numbers of SNP genotypes. In order to meet this need, while at the same time continuing to support the sequencing and microsatellite requirements of the scientists we serve, the Core needs to update its capacity. We are requesting funds for an AB 3730XL, 96 Capillary DNA Analyzer. The Core has been successfully operating older models of this technology for over five years. Currently there are two AB 3700 capillary instruments, and one AB 3730S, 48 capillary instrument. These three instruments are currently running close to capacity, generating DNA sequence, microsatellites, and SNPs on a daily basis. The 3700 instruments have been in continuous operation for four years, and will need to be replaced very soon. The 3700 cannot run SNP genotypes, and the sequence it produces has shorter read lengths and inferior quality to the 3730. The 3700 is less efficient, more prone to break-down, and has higher running costs and per-sample costs than the 3730. The model has been discontinued, and will not be supported within two years of the requested funding period. Therefore, it is necessary to replace the 3700s with the requested 3730 instrument.

该子项目是利用 NIH/NCRR 资助的共享仪器补助金提供的资源的众多研究子项目之一。子项目和研究者 (PI) 可能已从另一个 NIH 来源获得主要资金，因此可以在其他 CRISP 条目中出现。列出的机构是资助机构，不一定是研究者的机构。描述（由申请人提供）：通过大型关联研究、大量样本和 SNP 对复杂性状的研究推动了基因分型的需求迅速增长。与此同时，对微卫星基因分型和测序的需求仍然至少以目前的水平存在。因此，必须提高快速、廉价、高通量测序和基因分型的能力。最近实验室仪器的快速发展完全能够满足这种不断增长的需求。然而，这种高科技的价格也很高。事实证明，除了最大、资金最充足的实验室之外，随着研究的进展，任何实验室都不可能找到资源从一种尖端技术转向另一种尖端技术。为了解决这个问题，加州大学洛杉矶分校承诺通过提供最先进的核心设施系统来支持和促进所有科学家的研究。这些核心的人员配备和装备使科学家能够将更多时间集中在解决智力问题上，而不是花在获取和学习新技术上。加州大学洛杉矶分校测序和基因分型核心可满足当地科学界大量用户的研究需求。我们已经确定未来需要一种廉价的方法来产生越来越多的 SNP 基因型。为了满足这一需求，同时继续支持我们所服务的科学家的测序和微卫星需求，核心需要更新其能力。我们正在申请资金购买 AB 3730XL、96 毛细管 DNA 分析仪。 Core 已成功运行该技术的旧型号已有五年多了。目前有两台 AB 3700 毛细管仪器和一台 AB 3730S，48 毛细管仪器。这三台仪器目前已接近满负荷运行，每天都会生成 DNA 序列、微卫星和 SNP。 3700 仪器已连续运行四年，很快需要更换。 3700 无法运行 SNP 基因型，其产生的序列读长较短，质量比 3730 差。3700 效率较低，更容易崩溃，并且运行成本和每个样本成本比 3730 更高。该模型已停产，并且在请求的资助期限后两年内不会得到支持。因此，有必要将 3700s 替换为所要求的 3730 仪器。