Examination of Odorant Receptors in Anopheles gambiae

冈比亚按蚊气味受体的检测

• 批准号: 6682144
• 负责人: LAURENCE J ZWIEBEL
• 金额: $ 24.33万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-07-01 至 2007-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6682144
• 关键词: Anopheles; age difference; arrestins; arthropod genetics; behavioral /social science research tag; behavioral genetics; biological signal transduction; blood; chemoreceptors; disease vectors; eating; female; focus groups; gender difference; genetic screening; host organism interaction; laboratory mouse; malaria; male; neurons; olfactions; preference; receptor expression

DESCRIPTION (provided by applicant): Chemosensory responses are critical components that direct several essential behaviors of insects that are vectors for pathogens responsible for many important human diseases. In particular, olfaction plays a major role in host seeking and selection behavior of blood-feeding female mosquitoes and as such constitutes a critical component of the mosquito's ability to transmit diseases such as malaria, dengue, yellow fever and West Nile Nile virus encephalitis. Within this context, I have undertaken a molecular examination of several elements of the olfactory signal transduction cascade in the principal African malaria vector mosquito Anopheles gambiae sensu stricto, where a significant preference for human hosts (anthropophily) underlies its inherent ability to transmit human malaria. An increased understanding of olfactory mechanisms and their underlying chemical cues in this system may provide insight in the processes of insect behavioral responses in general and vector disease transmission in particular. Moreover, this study would likely be instrumental in the development of novel mosquito control strategies targeted against this broad family of disease vectors, as well as other insects that pose considerable medical and economic threats through their ability to transmit disease and to act as agricultural pests. The objectives of this proposal complements ongoing studies in the laboratory of olfactory arrestins in A.gambiae and focus on an examination of a family of candidate odorant receptor proteins in A. gambiae (AgORs) that has recently been identified by this laboratory. Thus far, we have identified 44 candidate AgORs and have performed an initial characterization of a small subset of this gene family. While the exact number of potential AgORs remain to be established in A. gambiae, we proposed to begin a systematic analysis of this gene family by completing several studies focusing on their expression in the mosquito. Initially, in order to establish a strategic plan for future studies, we will employ a variety of molecular approaches to identify AgOR family members that exhibt sex-specific expression patterns. These AgORs will form our primary focus group for subsequent characterization of the developmental, spatial and temporal expression pattems of AgORs. In addition, in an extension of initial studies that specifically targets AgOR's role in the olfactory processes that underlie disease transmission, will we propose to conduct a series of detailed experiments to examine the temporal kinetics of AgOR expression in response to blood feeding.

描述（由申请人提供）：化学感应反应是关键组成部分，它们指导昆虫的几种基本行为，这些行为是导致许多重要人类疾病的病原体的载体。尤其是，嗅觉在寻求和选择喂养血液蚊子的宿主和选择行为中起着重要作用，因此构成了蚊子传播疾病的能力的关键组成部分，例如疟疾，登革热，黄热病和西尼罗河尼罗河病毒病毒。在这种情况下，我对主要的非洲疟疾媒介蚊子蚊子gambiae sensu stricto进行了嗅觉信号转导级联的几个元素进行分子检查，其中对人类宿主（拟人化）的重大偏爱是其固有的传播人类疟疾的固有能力。对该系统中嗅觉机制及其基本化学提示的越来越多的理解可能会在一般情况下，尤其是媒介疾病传播的昆虫行为反应过程中提供洞察力。此外，这项研究可能有助于针对这种广泛的疾病媒介的新型蚊子控制策略的发展，以及其他通过传播疾病并充当农业害虫的能力而构成相当大的医学和经济威胁的昆虫。该提案的目标补充了在A.gambiae的嗅觉阻止蛋白实验室正在进行的研究，并着重于对该实验室最近已经确定的甘比亚（A. Agors）的候选气味受体蛋白的检查。到目前为止，我们已经确定了44个候选剂，并对该基因家族的一小部分进行了初始表征。尽管在冈比亚葡萄球菌中仍有确切的潜在搅拌器数量，但我们提议通过完成几项重点介绍其在蚊子中的表达的研究开始对该基因家族进行系统的分析。最初，为了制定未来研究的战略计划，我们将采用各种分子方法来识别表现出性别特定表达模式的Agor家族成员。这些agor将构成我们的主要焦点组，以随后表征搅拌器的发育，空间和时间表达曲折。此外，在最初的研究的扩展中，该研究专门针对Agor在传播疾病传播的嗅觉过程中的作用，我们将建议进行一系列详细的实验，以检查Agor表达的时间动力学，以响应血液喂养。