ROLES OF HOX GENES IN SKIN & HAIR FOLLICLE DEVELOPMENT

HOX 基因在皮肤中的作用

• 批准号: 6650391
• 负责人: ALEXANDER AWGULEWITSCH
• 金额: $ 26.88万
• 依托单位: MEDICAL UNIVERSITY OF SOUTH CAROLINA
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-08-10 至 2006-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6650391
• 关键词: cell proliferation; developmental genetics; genetic mapping; genetic regulation; genetically modified animals; hair follicle; histogenesis; histopathology; homeobox genes; keratinocyte; laboratory mouse; northern blottings; skin; skin hyperplasia; subtraction hybridization

DESCRIPTION: (Adapted from applicant's abstract) - The distinct expression patterns of many members of the phylogenetically conserved homeobox ("HOX") gene family in both developing and adult skin implicate this group of controller genes in patterning and regenerative processes of the skin. The principal investigator has preliminary data that transgenic mice over-expressing Hoxc13 (GC13 mice) in pre- and postnatal skin exhibit hyperproliferation of follicular and epidermal keratinocytes. These mice exhibit alopecia and a progressive skin defect resembling ichthyosis. This animal model will be used to define the molecular mechanisms underlying these abnormalities, by studying expression of Hoxc13 and its neighbor Hoxc13, screening for differentially expressed mRNAs in skin of normal versus Hoxc13-mutant mouse skin, comparison of Hox12 knockout versus dominant gain-of-function mice, and transcriptional regulation of Hoxc13 and Hoxc12.

描述：（改编自申请人的摘要）- 独特的表达 系统发育保守同源盒（“HOX”）的许多成员的模式 发育中和成人皮肤中的基因家族都涉及这组 皮肤模式和再生过程中的控制基因。这 首席研究员初步数据表明转基因小鼠 产前和产后皮肤过度表达 Hoxc13（GC13 小鼠） 毛囊和表皮角质形成细胞过度增殖。这些老鼠 表现出脱发和类似鱼鳞病的进行性皮肤缺损。这 动物模型将用于定义这些背后的分子机制 异常，通过研究 Hoxc13 及其邻居 Hoxc13 的表达， 筛选正常皮肤和皮肤中差异表达的 mRNA Hoxc13 突变小鼠皮肤，Hox12 敲除与显性的比较 功能获得小鼠，以及 Hoxc13 和 Hoxc12 的转录调控。