IN VIVO EXPERIMENTATION CORE

体内实验核心

• 批准号: 6949486
• 负责人: ALEXANDER AWGULEWITSCH
• 金额: $ 13.56万
• 依托单位: MEDICAL UNIVERSITY OF SOUTH CAROLINA
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-01 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6949486
• 关键词: animal colony; biomedical facility; breast neoplasms; disease /disorder model; genetically modified animals; laboratory mouse

The purpose of Core B, the In Vivo Experimentation Core (IVEC) is to provide logistical support, expertise and guidance for examining Ets gene function in vivo by using the mouse as model organism. This core consists of five components, including, (i) maintenance and husbandry of transgenic mouse strains, (ii) generation of new transgenic models, (iii) morphometrical and histological analysis of mammary gland (iv) cryopreservation, and (v) reporter gene analysis. This core structure ensures access to state-of-the-art technology to for studying mammary gland tumor development and metastasis in existing and new transgenic mouse models. Centralizing in vivo experimentation has several distinct advantages over individualized experimentation, including greater efficiency, uniformity of experimental conditions (which facilitates direct data comparison), providing a source of inspiration for developing plans or hypotheses for cross-project experimentation (e.g. crossing of mutant strains), and rapid adaptation to emerging new methodologies. Consequently, this core will be a dynamic unit with maximal responsiveness to the needs of the individual projects both at present, and as they arise during their progress.

核心 B（体内实验核心 (IVEC)）的目的是提供后勤支持、专业知识 并指导使用小鼠作为模型生物体内检查 Ets 基因功能。这个核心 由五个部分组成，包括：(i) 转基因小鼠品系的维护和饲养，(ii) 新转基因模型的产生，（iii）乳腺的形态测量和组织学分析（iv） 冷冻保存，以及 (v) 报告基因分析。这一核心结构确保获得最先进的技术 用于研究现有和新转基因乳腺肿瘤发展和转移的技术 鼠标模型。与个体化实验相比，集中式体内实验具有几个明显的优势 实验，包括更高的效率、实验条件的统一性（这有利于直接 数据比较），为跨项目制定计划或假设提供灵感来源 实验（例如突变株的杂交）以及快速适应新兴的新方法。 因此，该核心将成为一个动态单元，能够最大程度地响应个人的需求 当前的项目以及在其进展过程中出现的项目。