Automated Pap Screen with HPV and Multispectral Imaging

采用 HPV 和多光谱成像的自动巴氏筛查

• 批准号: 6694889
• 负责人: ELLIOT S WACHMAN
• 金额: $ 10万
• 依托单位: CHROMODYNAMICS, INC. (CDI)
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-07-01 至 2005-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6694889
• 关键词: bioimaging /biomedical imaging; biomedical automation; biomedical equipment development; cervical /vaginal smear; cervix neoplasms; diagnosis design /evaluation; human papillomavirus; imaging /visualization /scanning; monoclonal antibody; neoplasm /cancer diagnosis; optics

DESCRIPTION (provided by applicant): The standard of care for cervical cancer screening is the Papanicolaou (Pap) smear. Manual review of smears is extremely labor intensive, and present commercial automated systems have significant limitations. We propose to address these deficiencies with a novel approach to automated Pap screening that uses high-risk HPV immunofluorescence labeling together with Pap staining as well as multispectral imaging and texture analysis. The HPV fluorescence signal will be used for a low magnification survey of the slide. Regions that show fluorescence will then be examined at high magnification using multispectral imaging. In this project we will: a) develop protocols to detect an HPV-16 gene product using a monoclonal antibody attached to a fluorescent probe in a slide that also contains Pap stain; b) develop an acousto-optic tunable filter based automated multispectral imaging station; c) develop analysis and classification algorithms to use this multispectral data to distinguish between cells that require clinical follow-up and those that do not. Validation of the technique will be performed using a set of HSIL cell samples together with a set of normal cervical cell samples as a control. Our goal is to obtain 100% sensitivity and 80% specificity on this initial study group.

描述（由申请人提供）： 宫颈癌筛查的护理标准是Papanicolaou（PAP）涂片。涂片的手动审查是极限量的，目前的商业自动化系统具有重大局限性。我们建议通过一种新型的自动PAP筛选方法来解决这些缺陷，该方法使用高风险的HPV免疫荧光标签以及PAP染色以及多光谱成像和纹理分析。 HPV荧光信号将用于对载玻片的低放大调查。 然后，将使用多光谱成像在高放大倍率下检查显示荧光的区域。在此项目中，我们将：a）使用附着在载玻片上的荧光探针上的单克隆抗体来开发协议来检测HPV-16基因产物，该抗体也包含PAP染色； b）开发一个基于声学的可调滤波器的自动多光谱成像站； c）开发分析和分类算法，以使用此多光谱数据来区分需要临床随访的细胞和不进行临床随访的细胞。 该技术的验证将使用一组HSIL细胞样品以及一组正常的宫颈细胞样品作为对照进行。我们的目标是在此初始研究组中获得100％灵敏度和80％的特异性。