Periplasmic amidases in bacterial cell division.

细菌细胞分裂中的周质酰胺酶。

• 批准号: 2097254
• 金额: --
• 依托单位: University of Warwick
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2018
• 资助国家: 英国
• 起止时间: 2018 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2097254
• 关键词: Periplasmic; amidases; bacterial; cell; division.

During division, bacteria must break and rebuild the peptidoglycan wall that envelopes their cytoplasmic membrane to enable segregation of daughter cells. In E. coli, the enzymes responsible for breaking peptidoglycan during division are periplasmic amidases that are regulated by a specialised ABC transporter-like protein (FtsEX) and its cognate adaptor protein (EnvC).This project will address the regulation of periplasmic amidases associated with petidoglycan remodelling using a combination of X-ray crystallography and microbiological techniques. The key question to be answered is how periplasmic amidases interact with, and are activated by, FtsEX-EnvC. The project aims to provide deep molecular understanding of amidase activity during bacterial cell division that may be useful in developing future antibacterial agents.

在分裂过程中，细菌必须破坏并重建包裹其细胞质膜的肽聚糖壁，以实现子细胞的分离。在大肠杆菌中，在分裂过程中负责破坏肽聚糖的酶是周质酰胺酶，它们受到专门的 ABC 转运蛋白样蛋白 (FtsEX) 及其同源接头蛋白 (EnvC) 的调节。该项目将解决与以下相关的周质酰胺酶的调节结合 X 射线晶体学和微生物技术进行肽聚糖重塑。要回答的关键问题是周质酰胺酶如何与 FtsEX-EnvC 相互作用并被 FtsEX-EnvC 激活。该项目旨在提供对细菌细胞分裂过程中酰胺酶活性的深入分子理解，这可能有助于开发未来的抗菌剂。