Cholesterol and Hydroxycholesterol Shaping Phagocytosis

胆固醇和羟基胆固醇塑造吞噬作用

• 批准号: DP140101461
• 负责人: Prof Jennifer Stow
• 金额: $ 29.36万
• 依托单位: The University of Queensland
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2014
• 资助国家: 澳大利亚
• 起止时间: 2014-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP140101461
• 关键词: Cholesterol; Hydroxycholesterol; Shaping; Phagocytosis

Reports now show that membrane cholesterol and 25-hydroxycholesterol (25HC) are required for immune cells to ingest and kill pathogens by phagocytosis. This project will measure phagocytosis in macrophages with genetically or pharmacologically varied cholesterol and 25HC, to compare and quantify the ingestion of different bacteria, fungi and particles. This project will also address the link between cholesterol synthesis, its storage in lipid bodies and its availability for phagocytosis, based on preliminary data showing such defects in the staggerer mouse model. Notably, cholesterol dysregulation is now a prevalent condition in society and our results will reveal at a fundamental, molecular level how this might compromise immune defenses.

现在的报告表明，膜胆固醇和25-羟基胆固醇（25HC）是免疫细胞通过吞噬作用摄取和杀死病原体所必需的。该项目将测量巨噬细胞对遗传或药理学不同的胆固醇和 25HC 的吞噬作用，以比较和量化不同细菌、真菌和颗粒的摄入量。该项目还将基于显示交错小鼠模型中此类缺陷的初步数据，研究胆固醇合成、其在脂体中的储存及其可用于吞噬作用之间的联系。值得注意的是，胆固醇失调现在是社会中普遍存在的情况，我们的研究结果将在基本的分子水平上揭示这如何可能损害免疫防御。