Characterization/Inhibition of Tyr-DNA Phosphodiesterase

Tyr-DNA 磷酸二酯酶的表征/抑制

• 批准号: 6546202
• 负责人: AMY C RAYMOND
• 金额: $ 2.5万
• 依托单位: SAN DIEGO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-15 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6546202
• 关键词: DNA topoisomerases; X ray crystallography; analytical chemistry; chemical cleavage; chemical kinetics; combinatorial chemistry; enzyme mechanism; fluorimetry; gel mobility shift assay; high throughput technology; peptide library; phosphodiesterase inhibitors; phosphodiesterases; predoctoral investigator; tyrosine

DESCRIPTION (provided by applicant): Tyrosyl-DNA phosphodiesterase I hydrolyzes Topoisomerase Ib from substrate DNA. Tyrosyl-DNA Phosphodiesterase appears to be involved in replication-induces double strand DNA breaks and an attractive chemotherapeutic target. This project aims to characterize this enzyme and discover an inhibitory molecule by deconvoluting combinatorial libraries.

描述（由申请人提供）：酪氨酰-DNA磷酸二酯酶I水解底物DNA中的拓扑异构酶Ib。 酪氨酰 DNA 磷酸二酯酶似乎参与复制诱导双链 DNA 断裂，并且是一个有吸引力的化疗靶点。 该项目旨在表征这种酶并通过解卷积组合文库发现抑制分子。