Function and Structure of Bacterial RNA Ligase

细菌RNA连接酶的功能和结构

• 批准号: 7055545
• 负责人: AMY C RAYMOND
• 金额: $ 4.6万
• 依托单位: SLOAN-KETTERING INST CAN RESEARCH
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-01-01 至 2008-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7055545
• 关键词: X ray crystallography; bacterial proteins; enzyme structure; gram positive bacteria; phosphoester ligase; postdoctoral investigator; protein structure function

DESCRIPTION (provided by applicant): Our lab has recently identified a novel and functional RNA ligase in the bacterium Deinococcus radiodurans. Because homologous proteins can be found in several other organisms, we anticipate the finding of this research to translate to a broader biological phenomenon. Additionally, the gene encoding the Deinococcus RNA ligase on which this study focuses is located adjacent to other putative RNA repair pathway members. In the course of my proposed research I will carefull yexamine the substrate requirements for this new enzyme, determine the role of a novel domain that is conserved across genera but of unknown function, and identify residues that contribute to catalysis, as well as characterize the nature of their contributions. In addition to these in vitro experiments, I will also examine the role of this RNA ligase in vivo and use X-ray crystallography to determine the native structure.

描述（由申请人提供）：我们的实验室最近在耐辐射球菌中发现了一种新型的功能性 RNA 连接酶。由于同源蛋白质可以在其他几种生物体中找到，我们预计这项研究的发现将转化为更广泛的生物现象。此外，本研究重点关注的编码奇球菌 RNA 连接酶的基因位于与其他假定的 RNA 修复途径成员相邻的位置。在我提出的研究过程中，我将仔细检查这种新酶的底物要求，确定跨属保守但功能未知的新结构域的作用，并识别有助于催化的残基，并表征其性质他们的贡献。除了这些体外实验之外，我还将检查这种 RNA 连接酶在体内的作用，并使用 X 射线晶体学来确定天然结构。