THEORY OF MULTI PHOTON EXCITATION

多光子激发理论

• 批准号: 6444724
• 负责人: PATRIK R CALLIS
• 金额: $ 29.31万
• 依托单位: UNIVERSITY OF MARYLAND BALTIMORE
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-04-01 至 2002-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6444724
• 关键词: biological products; biomedical resource; informatics; spectrometry; technology /technique

Theoretical calculations of electronic transitions in organic molecules for different modes of excitation allow one to predict the cross-section and fundamental anisotropy for multi-photon excitation. The fundamental anisotropy of NADH is near 0.54 for two-photon excitation from 690-740 nm, which is 46% higher than the value of 0.37 observed for one-photon excitation. This observation of a higher anisotropy with two-photon excitation was consistent with INDO/SDCI calculation of the one- and two-photon transition. NADH displays a low cross-section for two photon excitation, which suggest that fluorescence from NADH will be negligible for two-photon fluorescence microscopy and will not significantly interfere with observations of TPIF of other extrinsic probes used to label cells.

有机电子过渡的理论计算 不同激发模式的分子可以预测 用于多光子激发的横截面和基本各向异性。 NADH的基本各向异性对于两光子而接近0.54 激发690-740 nm，比0.37高46％ 观察到单光子激发。 对较高的观察 带有两光激发的各向异性与Indo/SDCI一致 一单光子过渡的计算。 纳德（Nadh）显示a 两个光子激发的低横截面，这表明 来自NADH的荧光对于两光子荧光的荧光可以忽略不计 显微镜，不会显着干扰 用于标记细胞的其他外部探针的TPIF。