TWO-PHOTON STUDIES OF AMINO ACIDS AND NUCLEIC ACIDS

氨基酸和核酸的双光子研究

• 批准号: 3280162
• 负责人: PATRIK R CALLIS
• 金额: $ 15.05万
• 依托单位: MONTANA STATE UNIVERSITY - BOZEMAN
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-04-01 至 1991-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3280162
• 关键词: DNA; Raman spectrometry; benzene; chemical substitution; cyclic aminoacid; dinucleotide; electronic spectra; fluorescence polarization; fluorescence spectrometry; guanosine; indoles; molecular orbital; naphthalenes; nucleic acid structure; photoelectron spectrometry; polymers; ultraviolet spectrometry; virus DNA

Two-photon electronic state spectroscopy which offers selection rules complementary to those of ordinary UV-visible spectroscopy is used to study the low lying electronic singlet excited states of aromatic amino acids, DNA bases, nucleotides and DNA polymers. Studies of model aromatic systems are continued for the purpose of forming a firm bridge of understanding between substituted aromatics and the less well-understood indoles, purines and pyrimidine bases. INDO/S calculations, with emphasis on thorough investigation of basis set, parameter and geometry effects, will be applied to each experiment. The long range goal is to attain a firmly based understanding, with the aid of transition density pictures, of the electronic structure of these systems -- one that enjoys a similar degree of trust given to simple substituted benzenes. Some focus is on the specific aim of locating the "La origin" in the tryptophan chromaphore, indole; one-photon laser UV polarized fluorescence experiments on indoles in vapor and hydrocarbon glass are proposed to support parallel two-photon experiments. One-photon fluorescence as well as two-photon experiments on one and two-stranded viral DNA's are proposed, especially to see if intrinsic fluorescence depends on supercoiling and protein complexation. The intrinsic fluorescence of Z-DNA will be observed.

提供选择规则的两光子电子状态光谱 与普通紫外线可见光谱法的互补性用于研究 芳族氨基酸的低谎言电子单线激发态， DNA碱基，核苷酸和DNA聚合物。 模型芳香系统的研究 继续进行，目的是形成坚定的理解桥梁 在替代的芳香剂和较不熟悉的吲哚之间，嘌呤 和嘧啶碱。 印度计算，重点是彻底 将应用基础集，参数和几何效应的研究 每个实验。 远距离目标是实现一个牢固的基础 借助于过渡密度图片的理解 这些系统的电子结构 - 享有类似程度的电子结构 信任简单替代的苯。 一些重点是 在色氨酸Chromaphore中找到“ La Origin”的具体目的， 吲哚；吲哚上的单光子激光紫外线极化实验 提出在蒸气和碳氢化合物中以支持平行的两光子 实验。 一光子荧光以及两光子实验 提出了一个和两链病毒DNA，特别是查看是否是否 固有的荧光取决于超涂层和蛋白质络合。 将观察到Z-DNA的固有荧光。