CONNECTIONS OF LIMBIC SYSTEM & HYPOTHALAMUS

边缘系统的连接

• 批准号: 6477591
• 负责人: LARRY W SWANSON
• 金额: $ 4.85万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-08-01 至 2002-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6477591
• 关键词: Mammalia; bioimaging /biomedical imaging; biomedical resource; computers; magnetic resonance imaging; model design /development; nervous system; nuclear magnetic resonance spectroscopy; statistics /biometry

Our goal is to generate an easily accessible MR database of quantitative normal brain development in a species of non-human primate closely related to the human. We plan to define age specific normal ranges of intersubject variability for each developmental measure for use in future studies of abnormal brain development patterned after human neuroanatomic and psychobehavioral disease. We will integrate quantitative imaging knowledge of normal brain development with biochemical, histologic and behavioral data available in the same animal. Specific Aims: 1. Use a thin section. high resolution. coronal 3D volume SPGR sequence to noninvasively quantify structural changes in selected neural areas. Using computer assisted volumetric analysis and segmentation techniques we will measure the volume and visualize changes in brain shape which occur in specific cerebral structures of the developing vervet monky. 2. Use the information Inherent in the MR sequence above and from inversion recovery sequences to quantify regional myelinated white matter. Short TR, short TE sequences are sensitive to myelinated white matter and will be used in the same population using the same experimental design as in 1. above. 3. To validate specific aims 1. and 2. by performing a quantitative histologic study of age-matched cryomycrotomed vervet brain sections using a common anatomic coordinate system. The developing brain is composed of distinct processing regions, which mature in an organized sequential pattern. The pattern of growth and mvelination of major brain regions in the vervet monkey has not been described. Recently developed computer assisted segmentation protocols have improved quantitative structural analysis of both the human and primate brain. These approaches rely on intensity discrimination and edge detection The approaches assume a direct relationship between pixel intensity and physical substrate. A thin slice, short time to echo (TE), short time to repetition (TR), gradient recalled acquisition in the steady state (SPGR) sequence using a high field 3.OT magnet can provide a low noise, high resolution three dimensional data set for reconstruction of a variety of brain structures. This will allow longitudinal intra-subject evaluation of the rate and regional pattern of brain development as determined by volume changes in specific neural areas.

我们的目标是生成一个易于访问的MR数据库 非人类物种中的定量正常脑发育 灵长类动物与人类密切相关。 我们计划定义年龄的特定年龄 每种发育的受试者间变异性的正常范围 措施用于未来关于异常大脑发育的研究 以人类神经解剖学和精神病性疾病为例。 我们 将整合正常大脑的定量成像知识 可用的生化，组织学和行为数据开发 在同一只动物中。 具体目的： 1。使用薄部分。 高分辨率。 Coronal 3D卷SPGR 无创的顺序量化所选的结构变化 神经区域。 使用计算机辅助体积分析和 分割技术我们将测量体积并可视化 大脑形状的变化，发生在特定的脑结构中 发展中的Vervet Monky。 2。使用该信息中固有的信息 MR序列上方和从反转恢复序列进行量化 区域髓载的白质。 短tr，短te序列是 对髓白质敏感，将在同一中使用 种群使用与上述1相同的实验设计。 3 通过执行定量来验证特定目标1和2。 年龄匹配的冰冻杂交脑部的组织学研究 使用通用的解剖坐标系。 发育中的大脑由不同的处理区组成， 在有组织的顺序模式中成熟。 增长模式和 Vervet Monkey中主要大脑区域的污染尚未 描述。 最近开发了计算机辅助细分 协议已改进了两者的定量结构分析 人类和灵长类动物的大脑。 这些方法取决于强度 歧视和边缘检测方法假定直接 像素强度与物理基材之间的关系。 瘦 切片，回声（TE）的时间短，重复时间短（TR）， 梯度召回稳态（SPGR）序列的采集 使用高场3.ot磁铁可以提供低噪声，高 分辨率三维数据集用于重建一种 大脑结构。 这将允许纵向内部受试者 评估大脑发育的速率和区域模式 由特定神经区域的体积变化确定。