CONNECTIONS OF THE LIMBIC SYSTEM AND HYPOTHALAMUS

边缘系统和下丘脑的连接

• 批准号: 8171160
• 负责人: LARRY W SWANSON
• 金额: $ 1.22万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-01 至 2011-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8171160
• 关键词: Aggressive behavior; Anorexia; Anxiety; Appetite Regulation; Back; Behavior; Blood Glucose; Brain; Brain Part; Brain Stem; Cardiovascular system; Cerebral hemisphere; Chemistry; Clinical; Computer Retrieval of Information on Scientific Projects Database; Data; Diffuse; Dorsal; Emotions; Endocrine; Food; Funding; Genes; Grant; Histocytochemistry; Hunger; Hypothalamic structure; Individual; Institution; Internet; Lateral; Limbic System; Measures; Medial; Mediating; Menstrual cycle; Methods; Moods; Neuraxis; Neurons; Neurotransmitters; Obesity; Output; Pathology; Pathway interactions; Pattern; Phase; Physiology; Pituitary Gland; Rattus; Recording of previous events; Reproductive Behavior; Research; Research Personnel; Resolution; Resources; Sex Behavior; Sleep; Sleep Wake Cycle; Source; Spinal Cord; Structure; System; Thirst; United States National Institutes of Health; Wakefulness; Work; base; cell type; design; fighting; neural circuit; neuroinformatics; reproductive

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The long-term objective of the proposed work is to identify and characterize at high resolution the structure and chemistry of neural circuits in the mammalian brain that underlie the basic drives and emotions keeping individuals alive and assuring survival of the species associated with hunger and thirst, defensive (fight or flight) behavior, reproductive (sexual and parental) behavior, and the sleep/wake cycle. Previous work identified the hypothalamus as a critical node in this circuitry, and characterized in detail axonal input/output relationships of its medial half with the pituitary gland, cerebral hemispheres, and brainstem/spinal cord. The proposed work is designed to complete in a systematic way, and to extend, a similar analysis of the lateral hypothalamus, and is based on the hypothesis that the lateral hypothalamus consists of two fundamentally different components, a dorsal region with widespread, diffuse projections to much of the central nervous system, and a surrounding compartmentalized region divided into some two dozen parts with very specific projection patterns to specific functional systems. The three specific aims are, a) to complete a phase one, regional analysis of the surrounding compartmentalized region; b) to begin a phase two, neuronal cell type analysis, starting with certain lateral hypothalamic regions apparently involved critically in food appetite regulation; and c) to facilitate the entry of neuroanatomical data into neuroinformatics workbenches on the web. The research is based on the combined use in rats of experimental intra-axonal pathway tracing methods, and hybridization histochemistry for the cell type-specific expression of neurotransmitter-related genes. There is a rich clinical and experimental history, dating back over a century, that disturbances of the hypothalamus, and parts of the brain interacting with the hypothalamus, produce depending on localization either obesity or anorexia, profound effects on cardiovascular and endocrine (for example, blood glucose) measures, anxiety and mood, aggressive behavior, sexual behavior and physiology (for example, the menstrual cycle), and disturbances of normal sleep and wakefulness patterns. The proposed research is designed to clarify very poorly understood neural circuits that mediate normal ingestive, defensive, reproductive, and sleep/wake behaviors; understanding their pathology will follow.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 拟议工作的长期目标是以高分辨率识别和表征哺乳动物大脑中神经回路的结构和化学，这些神经回路是维持个体生存并确保与饥饿和口渴相关的物种生存的基本驱动和情绪的基础，防御（战斗或逃跑）行为、生殖（性和养育）行为以及睡眠/觉醒周期。先前的工作将下丘脑确定为该回路中的关键节点，并详细描述了其内侧半部与垂体、大脑半球和脑干/脊髓的轴突输入/输出关系。拟议的工作旨在以系统的方式完成并扩展对外侧下丘脑的类似分析，并且基于这样的假设：外侧下丘脑由两个根本不同的部分组成，一个具有广泛的、弥漫性投影的背侧区域中枢神经系统的大部分，以及周围的划分区域，分为大约两打部分，具有非常特定的投射模式到特定的功能系统。这三个具体目标是：a) 完成第一阶段对周围分区区域的区域分析； b) 开始第二阶段的神经元细胞类型分析，从某些明显参与食物食欲调节的外侧下丘脑区域开始； c) 促进神经解剖学数据输入网络上的神经信息学工作台。该研究基于在大鼠中结合使用实验性轴突内通路追踪方法和杂交组织化学来检测神经递质相关基因的细胞类型特异性表达。一个多世纪以来，丰富的临床和实验历史表明，下丘脑以及与下丘脑相互作用的大脑部分的紊乱，根据定位产生肥胖或厌食，对心血管和内分泌产生深远影响（例如，血糖）测量、焦虑和情绪、攻击行为、性行为和生理（例如月经周期）以及正常睡眠和觉醒模式的干扰。拟议的研究旨在澄清人们知之甚少的调节正常摄取、防御、生殖和睡眠/觉醒行为的神经回路；了解他们的病理学就会随之而来。