CONNECTIONS OF THE LIMBIC SYSTEM AND HYPOTHALAMUS

边缘系统和下丘脑的连接

• 批准号: 7724531
• 负责人: LARRY W SWANSON
• 金额: $ 1.03万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-08-01 至 2009-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7724531
• 关键词: Aggressive behavior; Animals; Arousal; Atlases; Axonal Transport; Behavior; Behavioral; Brain; Cell Nucleus; Cells; Chemistry; Classification; Computer Graphics; Computer Retrieval of Information on Scientific Projects Database; Databases; Disease; Dorsal; Eating; Funding; Goals; Grant; Hunger; Hypothalamic structure; In Situ Hybridization; Individual; Institution; Lateral Hypothalamic Area; Limbic System; Medial; Mediating; Methods; Mood Disorders; Neurons; Neurotransmitters; Output; Phenotype; Play; Positive Reinforcements; Prosencephalon; Rattus; Reproduction; Reproductive Behavior; Research; Research Personnel; Resolution; Resources; Role; Sleep Wake Cycle; Source; Structure; System; Telencephalon; Thirst; United States National Institutes of Health; Work; base; cell type; design; drinking; insight; mRNA Expression; motivated behavior; relating to nervous system; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The long-term goal of the proposed work is to characterize at high resolution the structure and chemistry of circuits in the mammalian brain that underlie the basic drives that keep individual animals alive and assure perpetuation of the species associated with hunger and thirst, defensive behavior, reproductive behavior, and the sleep/wake cycle. Previous neuroanatomical work has clarified the input/output relationships and neurotransmitters of medial hypothalamic subsystems mediating each of these broadly defined functions, and has shown that they-along with closely associated limbic telencephalon regions-project topographically to the lateral hypothalamic area (LHA). Functional evidence suggests that the LHA plays an important role in positive reinforcement, behavioral arousal, and various aspects of motivated behavior, although the topographic organization of its neural inputs and axonal projects is poorly understood. The proposed work is based on the hypotheses that the LHA has two functionally and structurally distinct zones related to behavioral state, and the ventral zone being divided into 20 or more distinct cell groups or nuclei related to specific functional systems. Three specific aims based on the use of experimental neuroanatomical methods in the rat are designed to explore this hypotheses: (1) A systematic computer graphics atlas/database of LHA differentiation based on connections and in situ hybridization-related mRNA expression will be elaborated. (2) Projections of cell groups in the dorsal and ventral regions will be determined and compared. And (3) neuronal cell types (phenotypes) in these regions will be determined using combined axonal transport/hybridization histochemical methods. This analytical/synthetic approach is designed to provide a structural framework for the rational design of functional experiments, as well as provide new insights into, and eventually treatments for, diseases that involve forebrain neural systems controlling, eating, drinking, aggression and defense reproduction, the sleep-wake cycle, and presumably mood disorders as well.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 拟议工作的长期目标是在高分辨率上表征哺乳动物大脑中电路的结构和化学，这是使单个动物保持活力的基本驱动力的基础，并确保了与饥饿和口渴，防御行为，生殖行为以及睡眠/唤醒周期相关的物种的永久化。以前的神经解剖学工作已经阐明了介导这些广泛定义的功能的内侧下丘脑子系统的输入/输出关系和神经递质，并表明它们与与后期下丘脑区域（LHA）上的密切相关的端型端脑区域密切相关。功能证据表明，LHA在积极的强化，行为唤醒和动机行为的各个方面起着重要作用，尽管对其神经输入和轴突项目的地形组织的理解很少。提出的工作基于以下假设：LHA在功能和结构上具有两个与行为状态相关的区域，并且腹侧区域分为20个或更多不同的细胞组或与特定功能系统有关的细胞组或核。基于大鼠中实验性神经解剖学方法的使用的三个特定目的旨在探讨该假设：（1）基于连接和原位杂交相关的MRNA表达的系统计算机图形图集/LHA差异数据库将被阐述。 （2）将确定并比较背侧和腹侧区域中细胞组的投影。 （3）这些区域中的神经元细胞类型（表型）将使用轴突转运/杂交组织化学方法确定。这种分析/合成方法旨在为功能实验的合理设计提供一个结构框架，并为涉及前脑神经系统控制，饮食，饮食，侵略和防御繁殖，睡眠效果，睡眠循环以及大概是情绪障碍的疾病提供了新的见解，并最终对疾病进行治疗。