CONNECTIONS OF THE LIMBIC SYSTEM AND HYPOTHALAMUS

边缘系统和下丘脑的连接

• 批准号: 7724531
• 负责人: LARRY W SWANSON
• 金额: $ 1.03万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-08-01 至 2009-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7724531
• 关键词: Aggressive behavior; Animals; Arousal; Atlases; Axonal Transport; Behavior; Behavioral; Brain; Cell Nucleus; Cells; Chemistry; Classification; Computer Graphics; Computer Retrieval of Information on Scientific Projects Database; Databases; Disease; Dorsal; Eating; Funding; Goals; Grant; Hunger; Hypothalamic structure; In Situ Hybridization; Individual; Institution; Lateral Hypothalamic Area; Limbic System; Medial; Mediating; Methods; Mood Disorders; Neurons; Neurotransmitters; Output; Phenotype; Play; Positive Reinforcements; Prosencephalon; Rattus; Reproduction; Reproductive Behavior; Research; Research Personnel; Resolution; Resources; Role; Sleep Wake Cycle; Source; Structure; System; Telencephalon; Thirst; United States National Institutes of Health; Work; base; cell type; design; drinking; insight; mRNA Expression; motivated behavior; relating to nervous system; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The long-term goal of the proposed work is to characterize at high resolution the structure and chemistry of circuits in the mammalian brain that underlie the basic drives that keep individual animals alive and assure perpetuation of the species associated with hunger and thirst, defensive behavior, reproductive behavior, and the sleep/wake cycle. Previous neuroanatomical work has clarified the input/output relationships and neurotransmitters of medial hypothalamic subsystems mediating each of these broadly defined functions, and has shown that they-along with closely associated limbic telencephalon regions-project topographically to the lateral hypothalamic area (LHA). Functional evidence suggests that the LHA plays an important role in positive reinforcement, behavioral arousal, and various aspects of motivated behavior, although the topographic organization of its neural inputs and axonal projects is poorly understood. The proposed work is based on the hypotheses that the LHA has two functionally and structurally distinct zones related to behavioral state, and the ventral zone being divided into 20 or more distinct cell groups or nuclei related to specific functional systems. Three specific aims based on the use of experimental neuroanatomical methods in the rat are designed to explore this hypotheses: (1) A systematic computer graphics atlas/database of LHA differentiation based on connections and in situ hybridization-related mRNA expression will be elaborated. (2) Projections of cell groups in the dorsal and ventral regions will be determined and compared. And (3) neuronal cell types (phenotypes) in these regions will be determined using combined axonal transport/hybridization histochemical methods. This analytical/synthetic approach is designed to provide a structural framework for the rational design of functional experiments, as well as provide new insights into, and eventually treatments for, diseases that involve forebrain neural systems controlling, eating, drinking, aggression and defense reproduction, the sleep-wake cycle, and presumably mood disorders as well.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 这项工作的长期目标是以高分辨率表征哺乳动物大脑中电路的结构和化学，这些电路是维持个体动物生存的基本驱动力，并确保与饥饿和口渴、防御行为、生殖行为和睡眠/觉醒周期。先前的神经解剖学工作已经阐明了调节这些广泛定义的功能的内侧下丘脑子系统的输入/输出关系和神经递质，并表明它们与密切相关的边缘端脑区域一起在地形上投射到外侧下丘脑区域（LHA）。功能证据表明，LHA 在积极强化、行为唤醒和动机行为的各个方面发挥着重要作用，尽管其神经输入和轴突项目的拓扑组织知之甚少。拟议的工作基于这样的假设：LHA 有两个与行为状态相关的功能和结构不同的区域，腹侧区域被分为 20 个或更多与特定功能系统相关的不同细胞群或细胞核。基于在大鼠中使用实验神经解剖学方法的三个具体目标旨在探索这一假设：（1）将阐述基于连接和原位杂交相关 mRNA 表达的 LHA 分化的系统计算机图形图谱/数据库。 (2) 确定并比较背侧和腹侧区域细胞群的投影。 (3) 这些区域中的神经元细胞类型（表型）将使用轴突运输/杂交组织化学组合方法来确定。这种分析/综合方法旨在为功能实验的合理设计提供结构框架，并为涉及前脑神经系统控制、饮食、攻击和防御繁殖的疾病提供新的见解和最终的治疗方法，睡眠-觉醒周期，大概还有情绪障碍。