CORE--CELL CULTURE

核心--细胞培养

• 批准号: 6493085
• 负责人: WILLIAM E SWEENEY
• 金额: $ 16.2万
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-01 至 2002-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6493085
• 关键词: autosomal recessive trait; biomedical facility; cell line; human tissue; laboratory mouse; phenotype; polycystic kidney

Description: (Taken directly from the application) The Cell Culture Core is a critical component of the Interdisciplinary Center for Polycystic Kidney Disease Research. It will provide and maintain primary cells and cell lines from human and murine control and cystic kidneys for in vitro experimentation by the eight center investigators (Project Leaders and Co-Investigators of the-3 major and two Pilot and Feasibility studies proposed). It will be the central resource for distribution of normal and cystic renal epithelial cell (REC) lines from the relevant models. This Core will concentrate expertise in the isolation of primary and immortalized (REC), maintenance and characterization of these cell lines, preparation of culture medium and cryopreservation of the cells. The goal of the Cell Culture Core is to provide investigators with high quality, phenotypically consistent cell lines in a cost-effective and efficient manner. In addition, the Core will provide Center Investigators with expertise in the utilization of organ culture methodology for specific studies proposed. Center personnel will be responsible for quality control within the facility. They will closely monitor the REC lines for phenotypic drift and perform periodic screening for contamination (i.e., mycoplasma). The Cell Core centralizes the isolation and handling of the primary cells and REC lines thus ensuring consistent reagents and minimizing phenotypic drift of cells being studied under various experimental protocols. The Core concentrates expertise in commonly utilized techniques thereby maximizing quality, efficiency, and cost effectiveness.

描述：（直接取自应用程序）细胞培养核心是多囊肾病研究跨学科中心的重要组成部分。它将提供和维护来自人类和小鼠对照以及囊性肾的原代细胞和细胞系，供八名中心研究人员（提议的 3 个主要项目的项目负责人和联合研究人员以及两项试点和可行性研究）进行体外实验。它将成为分配相关模型的正常和囊性肾上皮细胞（REC）系的中心资源。该核心将集中在原代和永生化 (REC) 的分离、这些细胞系的维护和表征、培养基的制备以及细胞的冷冻保存方面的专业知识。细胞培养核心的目标是以经济高效的方式为研究人员提供高质量、表型一致的细胞系。此外，核心将为中心研究人员提供利用器官培养方法进行具体研究的专业知识。中心人员将负责设施内的质量控制。他们将密切监测 REC 系的表型漂移，并定期筛查污染（即支原体）。 Cell Core 集中了原代细胞和 REC 细胞系的分离和处理，从而确保试剂的一致性并最大限度地减少在各种实验方案下研究的细胞的表型漂移。该核心集中了常用技术的专业知识，从而最大限度地提高质量、效率和成本效益。