CORE--CELL CULTURE

核心--细胞培养

• 批准号: 6493085
• 负责人: WILLIAM E SWEENEY
• 金额: $ 16.2万
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-01 至 2002-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6493085
• 关键词: autosomal recessive trait; biomedical facility; cell line; human tissue; laboratory mouse; phenotype; polycystic kidney

Description: (Taken directly from the application) The Cell Culture Core is a critical component of the Interdisciplinary Center for Polycystic Kidney Disease Research. It will provide and maintain primary cells and cell lines from human and murine control and cystic kidneys for in vitro experimentation by the eight center investigators (Project Leaders and Co-Investigators of the-3 major and two Pilot and Feasibility studies proposed). It will be the central resource for distribution of normal and cystic renal epithelial cell (REC) lines from the relevant models. This Core will concentrate expertise in the isolation of primary and immortalized (REC), maintenance and characterization of these cell lines, preparation of culture medium and cryopreservation of the cells. The goal of the Cell Culture Core is to provide investigators with high quality, phenotypically consistent cell lines in a cost-effective and efficient manner. In addition, the Core will provide Center Investigators with expertise in the utilization of organ culture methodology for specific studies proposed. Center personnel will be responsible for quality control within the facility. They will closely monitor the REC lines for phenotypic drift and perform periodic screening for contamination (i.e., mycoplasma). The Cell Core centralizes the isolation and handling of the primary cells and REC lines thus ensuring consistent reagents and minimizing phenotypic drift of cells being studied under various experimental protocols. The Core concentrates expertise in commonly utilized techniques thereby maximizing quality, efficiency, and cost effectiveness.

描述：（直接从应用程序取）细胞培养核心是多囊性肾脏疾病研究中心跨学科中心的关键组成部分。它将提供并维护来自人类和鼠控制和囊性肾脏的原代细胞和细胞系，用于八个中心研究者的体外实验（3大专业的项目负责人和共同投资者，以及两个试点和可行性研究）。它将是从相关模型中分布正常和囊性肾上皮细胞（REC）系的中心资源。该核心将集中专业知识，以分离初级和永生（REC），这些细胞系的维持和表征，制备培养基的制备以及细胞的冷冻保存。细胞培养核心的目的是以具有成本效益和有效的方式为研究人员提供高质量的表型一致细胞系。此外，核心将为中心研究人员提供针对特定研究的器官培养方法利用的专业知识。中心人员将负责设施内的质量控制。他们将密切监视表型漂移的REC线，并进行周期性筛查（即支原体）。细胞核心将原代细胞和REC系的分离和处理集中，从而确保在各种实验方案下研究的细胞的表型漂移一致，并最大程度地减少了表型漂移。核心集中在常用技术方面的专业知识，从而最大程度地提高了质量，效率和成本效益。