Physiology Core

生理学核心

基本信息

批准号：
10747627
负责人：
Marcelo Daniel Carattino
金额：
$ 28.79万
依托单位：
UNIVERSITY OF PITTSBURGH AT PITTSBURGH
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-09-01 至 2028-06-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10747627
关键词：
Address Animal Model Applications Grants Area Biological Assay Biological Models Biological Process Carrier Proteins Cell Line Cells Chemicals Collaborations Communities Core Facility Data Data Analyses Data Set Dedications Development Disease Electrophysiology (science)Ensure Enzymes Epithelial Cells Epithelium Equipment Experimental Designs Generations Genes Goals Health Human Imaging Device Imaging technology Institution Ion Transport Ions Journals Kidney Laboratories Light Maintenance Mammals Measurement Mediating Mediator Membrane Membrane Potentials Modeling Molecular Mus Nephrons Organ Organoids Oryctolagus cuniculus Perfusion Phenotype Physiological Physiological Processes Physiology Preparation Productivity Protein Analysis Proteins Protocols documentation Publications Quality Control Rattus Reagent Regulation Regulatory Pathway Renal function Reproducibility Research Research Personnel Resource Development Resources Science Series Signaling Molecule Structure System Systems Development Techniques Technology Time Tissues Training Transport Process Tubular formation Variant Work biomedical resource cell immortalization hands-on learning live cell imaging member novel protein expression quality assurance sabbatical sensor single molecule solute tool variant of unknown significance

项目摘要

Abstract The Physiology Core provides a series of graded technologies and expertise to investigators who wish to address fundamental questions about transport processes that occur in the tubular structures of the nephron and other relevant systems. To gain understanding of biological processes that mediate kidney function in health and disease states, in light of the phenotypic diversity and functional complexity of this organ, the use of strategies that allow specific nephron segments/cells/proteins to be studied in isolation under defined conditions is required. The Core, through its three Subcores, offers expertise and state-of-the-art technologies for studying the function and regulation of transport and other membrane resident proteins with progressive degrees of complexity from single molecules to model systems to native epithelia. The Single Tubule and Microperfusion Subcore provides tools and technologies to study the function and expression of transport proteins and associated regulatory pathways in single nephron segments isolated from small mammals and kidney organoids. The Electrophysiology Subcore provides expertise and a toolbox of electrophysiological approaches to study the function and regulation of transport proteins at the molecular and cellular levels in model systems and native epithelia. The Live-Cell Imaging Subcore offers imaging tools and technologies to assess ion transport, changes in membrane potential, cellular volume, intracellular mediators, and signaling molecules in immortalized cells and native preparations using chemical and genetically encoded fluorescent sensors. The Core will apply a multipronged approach and collaborate with the O’Brien Consortium to share specialized resources and technologies throughout the kidney research community, to lower the barrier to entry for early-stage investigators and researchers new to the kidney field, and to advance kidney research and discovery nationwide.

抽象的生理核心为希望的调查人员提供了一系列分级技术和专业知识解决有关肾单位结构中发生的运输过程的基本问题和其他相关系统。了解介导肾脏功能的生物学过程鉴于该器官的表型多样性和功能复杂性，健康和疾病状态允许特定的肾单位段/细胞/蛋白质的策略在定义下孤立研究需要条件。核心通过其三个子核提供了专业知识和最先进的技术用于研究运输和其他膜居民蛋白质的功能和调节从单分子到模型系统到天然上皮的复杂程度。单小管和微灌注子核提供了研究运输功能和表达的工具和技术从小型哺乳动物和肾脏器官。电生理子核提供了专业知识和电生理工具箱研究在分子和细胞水平的转运蛋白功能和调节的方法模型系统和本地上皮。实时成像子核提供成像工具和技术评估离子传输，膜电位的变化，细胞体积，细胞内介质和信号传导永生细胞中的分子和天然制剂使用化学和遗传编码的荧光传感器。核心将采用多管齐下的方法，并与O'Brien联盟合作分享整个肾脏研究社区的专业资源和技术，以降低障碍肾脏新手的早期研究人员和研究人员的入门，并推进肾脏研究和在全国范围内发现。