RESOURCE FOR ARRAYED BAC CLONES FOR FISH MAPPING OF TH

用于 TH 鱼类图谱的阵列 BAC 克隆资源

• 批准号: 6429902
• 负责人: Norma Jean Nowak
• 金额: $ 62.24万
• 依托单位: ROSWELL PARK CANCER INSTITUTE CORP
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-30 至 2003-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6429902
• 关键词: bacterial DNA; bacterial genetics; chromosomes; fluorescent in situ hybridization; genetic library; genetic mapping; genetic markers; molecular cloning; nucleic acid probes; nucleic acid sequence; oligonucleotides

DESCRIPTION: (Applicant's Description) Efforts supported through the Human Genome Project have resulted in the generation of both the clone resources and the technological developments required to interface the information produced through genome analysis and the clinical environment. As part of this effort, we have constructed large insert BAC (Bacterial Artificial Chromosome) libraries as a source of representative templates for sequencing the genome. Our current goal is the assembly of a genome wide BAC array at 1mb intervals to establish a framework of clones that can provide an immediate impact in cancer diagnostics genetics. This framework will be built on 4000 genetic and EST markers selected at the Sanger Center which have been ordered through the GB4, SG3, and TNG radiation panels, resulting in an optimal set of ordered markers available for assembling ale BAC array. The mapping information will be immediately available to the public through our website (http://bacpac.med.buffalo.edu) as well as the maps at NCBI/NLM. The individual and arrayed mapped clones will be distributed to the scientific community using our established distribution system, as well as, commercial resource companies. This BAC clone array will serve as nucleation points establishing the initial resource towards a whole genome BAC array that will have great future utility for techniques capable of scanning the entire genome in one experiment.

描述：（申请人的描述）人类支持的努力 基因组项目已经产生了克隆资源的产生 以及接口信息所需的技术发展 通过基因组分析和临床环境产生。 作为 这项工作，我们构建了大型插入BAC（细菌人造 染色体）库作为测序的代表模板的来源 基因组。 我们目前的目标是基因组宽BAC阵列的组装 1MB间隔以建立一个可以提供的克隆框架 直接影响癌症诊断遗传学。 这个框架将是 建立在Sanger中心选择的4000个遗传和EST标记的基础上 已通过GB4，SG3和TNG辐射面板订购，结果 在一组最佳的有序标记中，可用于组装啤酒BAC阵列。 映射信息将立即通过 我们的网站（http://bacpac.med.buffalo.edu）以及NCBI/NLM的地图。 个人和阵列映射的克隆将分配给 科学界使用我们既定的分销系统以及 商业资源公司。 这个BAC克隆阵列将作为 成核点建立了针对整个基因组的初始资源 BAC阵列将拥有能够有能力的技术的未来实用程序 在一个实验中扫描整个基因组。