Microarray & Genomics

微阵列

• 批准号: 7826845
• 负责人: Norma Jean Nowak
• 金额: $ 32.75万
• 依托单位: ROSWELL PARK CANCER INSTITUTE CORP
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7826845
• 关键词: Antibodies; Automation; Award; Bioinformatics; Biological Assay; Budgets; Cancer Center Support Grant; Cherry - dietary; Color; Complementary DNA; Contracts; Custom; Data Analyses; Development; Faculty; Foundations; Funding; Gene Expression; Gene Frequency; Generations; Genes; Genome; Genomic Library; Genomics; Genotype; Grant; Human; Human Genome Project; Hybridization Array; Image; Leadership; Libraries; Ligands; MALDI-TOF Mass Spectrometry; Maintenance; Methylation; Mus; Oligonucleotide Microarrays; Oligonucleotides; Peer Review; Preparation; Printing; Publications; RNA; Rattus; Recording of previous events; Research Infrastructure; Research Personnel; Resources; Robotics; Roswell Park Cancer Institute; SNP genotyping; Sampling; Scanning; Screening procedure; Services; Slide; Strategic Planning; Technology; Work; base; cDNA Library; cyanine dye 5; design; flexibility; high throughput technology; member; mouse genome; programs; technology development

The Microarray & Genomics Resource provides a full range of service in technologies for applied genomics. The importance of the Resource to the CCSG is reflected by a strong track record of utilization, publications and awarded grants and contracts. The Resource has a rich history of expertise and accomplishment in the Human Genome Project that laid the foundation for array technology development and analysis, development and implementation of high-throughput technologies and distribution of the RPCI BAC/PAC resources worldwide. The Microarray & Genomics Resource is composed of three complementary divisions: (1) The Genomics Division contributes to the application of human and mouse BAG clones for downstream functional analysis. It provides the infrastructure for automation through robotics for large-scale projects. Fifteen BAC/PAC genomic libraries and two cDNA libraries are available for screening, clone selection, characterization and distribution within RPCI. (2) The Microarray Division provides custom array design and analysis such as BAC-based array CGH for the human and mouse genomes, and gene expression, commercial platforms for two-color human, mouse and rat gene expression. Copy number studies are also available using Agilent and NimbleGen whole-genome oligonucleotide arrays. Investigators can request microarray services, including RNA/DNA isolation, amplification, Cy3 and Cy5 probe generation, hybridizations, custom-made cDNA, antibody, ligand and genomic arrays, scanning of microarrayed slides, image acquisition, normalization of data and analysis. (3) The Genotyping Division provides high-throughput genotyping of SNPs, SNP discovery and quantitative methylation services utilizing the Sequenom MALDI-TOF mass spectrometry platform. Investigators can request flexible assays designed for focused genotyping studies on large sample sets for as few as ten to as many as several hundred SNPs or genes. Over the project period, 54 CCSG Program members (43 with peer-reviewed funding) from five CCSG programs utilized the Resource. The Strategic Plan is to continue to provide access to state-of-theart technology facilitating large-scale and/or global analyses for applied genomics. The Director works closely with the CCSG leadership and members to guide, educate and support genomic endeavors. With the recruitment of new leadership and faculty in the CSBT, Til and MTET Programs, it is anticipated that CCSG members in all six CCSG programs will utilize this Resource. The Resource is used by five Programs and 46% of users are CCSG members. $121,671 in CCSG support is requested, representing 9% of the total operating budget.

微阵列和基因组学资源为应用提供全方位的技术服务 基因组学。资源对 CCSG 的重要性体现在良好的利用记录上， 出版物以及授予的赠款和合同。该资源拥有丰富的专业知识和历史 人类基因组计划的成就为阵列技术的发展奠定了基础 高通量技术的分析、开发和实施以及RPCI的分发 BAC/PAC 资源遍布全球。微阵列和基因组学资源由三个部分组成 互补部门： (1) 基因组学部门致力于人类和小鼠 BAG 克隆的应用 下游功能分析。它通过机器人技术为大规模自动化提供基础设施 项目。 15个BAC/PAC基因组文库和2个cDNA文库可用于筛选、克隆 RPCI 内的选择、表征和分配。 (2) 微阵列事业部提供定制阵列设计和分析，例如基于BAC的阵列 用于人类和小鼠基因组和基因表达的 CGH，用于双色人类的商业平台， 小鼠和大鼠的基因表达。还可以使用 Agilent 和 NimbleGen 进行拷贝数研究 全基因组寡核苷酸阵列。研究人员可以请求微阵列服务，包括 RNA/DNA 分离、扩增、Cy3 和 Cy5 探针生成、杂交、定制 cDNA、抗体、配体 和基因组阵列、微阵列载玻片扫描、图像采集、数据标准化和分析。 (3) 基因分型部门提供SNP的高通量基因分型、SNP发现和 利用 Sequenom MALDI-TOF 质谱平台进行定量甲基化服务。 研究人员可以要求针对大样本集进行集中基因分型研究而设计的灵活测定 少则十个，多则数百个 SNP 或基因。 在项目期间，来自 5 个国家的 54 名 CCSG 计划成员（43 名获得同行评审资金） CCSG 计划利用了该资源。该战略计划旨在继续提供最先进的技术 促进应用基因组学大规模和/或全球分析的技术。导演工作 与 CCSG 领导层和成员密切合作，指导、教育和支持基因组工作。和 在 CSBT、Til 和 MTET 项目中招聘新的领导层和教员，预计 所有六个 CCSG 计划的 CCSG 成员都将使用此资源。 该资源由五个计划使用，46% 的用户是 CCSG 成员。 CCSG 121,671 美元 请求支持，占总运营预算的 9%。