RESOURCE FOR ARRAYED BAC CLONES FOR FISH MAPPING OF TH

用于 TH 鱼类图谱的阵列 BAC 克隆资源

• 批准号: 2896754
• 负责人: Norma Jean Nowak
• 金额: $ 44.54万
• 依托单位: ROSWELL PARK CANCER INSTITUTE CORP
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-30 至 2003-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2896754
• 关键词: bacterial DNA; bacterial genetics; chromosomes; fluorescent in situ hybridization; genetic library; genetic mapping; genetic markers; molecular cloning; nucleic acid probes; nucleic acid sequence; oligonucleotides

DESCRIPTION: (Applicant's Description) Efforts supported through the Human Genome Project have resulted in the generation of both the clone resources and the technological developments required to interface the information produced through genome analysis and the clinical environment. As part of this effort, we have constructed large insert BAC (Bacterial Artificial Chromosome) libraries as a source of representative templates for sequencing the genome. Our current goal is the assembly of a genome wide BAC array at 1mb intervals to establish a framework of clones that can provide an immediate impact in cancer diagnostics genetics. This framework will be built on 4000 genetic and EST markers selected at the Sanger Center which have been ordered through the GB4, SG3, and TNG radiation panels, resulting in an optimal set of ordered markers available for assembling ale BAC array. The mapping information will be immediately available to the public through our website (http://bacpac.med.buffalo.edu) as well as the maps at NCBI/NLM. The individual and arrayed mapped clones will be distributed to the scientific community using our established distribution system, as well as, commercial resource companies. This BAC clone array will serve as nucleation points establishing the initial resource towards a whole genome BAC array that will have great future utility for techniques capable of scanning the entire genome in one experiment.

描述：（申请人的描述）通过人类支持的努力 基因组计划产生了两种克隆资源 以及信息交互所需的技术发展 通过基因组分析和临床环境产生。 作为一部分 为此，我们构建了大型插入 BAC（细菌人工 染色体）文库作为测序代表性模板的来源 基因组。 我们当前的目标是在以下位置组装全基因组 BAC 阵列： 1mb 间隔建立克隆框架，可以提供 对癌症诊断遗传学产生直接影响。 该框架将 基于桑格中心选择的 4000 个遗传和 EST 标记， 已通过 GB4、SG3 和 TNG 辐射板订购，结果 一组最佳的有序标记可用于组装 ale BAC 阵列。 测绘信息将立即通过以下方式向公众提供： 我们的网站 (http://bacpac.med.buffalo.edu) 以及 NCBI/NLM 的地图。 单个和阵列的映射克隆将被分发到 科学界使用我们既定的分配系统，以及， 商业资源公司。 该 BAC 克隆阵列将用作 成核点建立整个基因组的初始资源 BAC 阵列对于能够实现以下功能的技术具有巨大的未来实用性 在一项实验中扫描整个基因组。