STRUCTURE OF ENZYME SUBSTRATE COMPLEX FOR GENERATION OF UDP N ACETYLMURAMIC ACID

产生 UDP N 乙酰胞壁酸的酶底物复合物的结构

• 批准号: 6437582
• 负责人: JAMES M HOGLE
• 金额: $ 14.32万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-03-01 至 2002-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6437582
• 关键词: bacteria; bioimaging /biomedical imaging; biomaterials; biomedical resource; infection; radiography; structural biology

UDP-N-acetylenolpyruvylglucosamine reductase (MurB) is an enzyme in the peptidoglycan biosynthetic pathway of bacteria. It is responsible for the second committed step, namely the reduction of enolpyruvyl-UDP-N-acetylglucosamine (EP-UDPGlcNAc) to UDP-N-acetylmurammic acid (UDPMurNAc) by NADPH via a tightly bound flavin adenine dinucleotide cofactor. This resulting sugar is then the foundation for the addition of the five amino acids comprising the peptide chain and subsequently will be linked with N-acetylglucosamine to form the repeating disaccharide-pentapeptide subunit. Because of the essential nature of this enzyme in the synthesis of peptidoglycan to maintain osmotic integrity, this enzyme is a novel drug target for design of new antibacterial agents.

UDP-N-乙酰烯醇丙酮酰葡萄糖胺还原酶 (MurB) 是一种酶 在细菌的肽聚糖生物合成途径中。 这是 负责第二个承诺步骤，即减少 烯醇丙酮酰-UDP-N-乙酰葡糖胺 (EP-UDPGlcNAc) UDP-N-乙酰胞壁酸 (UDPMurNAc) 通过 NADPH 通过紧密结合 黄素腺嘌呤二核苷酸辅因子。 由此产生的糖是 添加五种氨基酸的基础 肽链，随后将与 N-乙酰氨基葡萄糖连接 形成重复的二糖五肽亚基。 由于 该酶在肽聚糖合成中的本质 为了维持渗透完整性，这种酶是一种新的药物靶点 新型抗菌剂的设计。