HIGH RESOLUTION CRYSTAL STRUCTURES OF BACTERIAL SODS

细菌 SODS 的高分辨率晶体结构

• 批准号: 6437525
• 负责人: KATRINA T FOREST
• 金额: $ 14.32万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-03-01 至 2002-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6437525
• 关键词: bacteria; bioimaging /biomedical imaging; biomaterials; biomedical resource; nervous system; proteins; radiography; structural biology

The bacterial Cu,Zn super oxide dismutases have a limited homology with the well-studied eukaryotic SOD enzymes, yet carry out the same chemical reaction. The seven critical active site histidines are maintained, as well as the metal ions, and are presumably in the same orientation as in the already known structures. The essential bacterial SOD's, with sequences so different from mammalian ones, are likely targets for therapeutic agents for many bacterial diseases. The enzymes for this project are from the human pathogen Neisseria meningitidis and the porcine pathogen Actinobacillus pleuropneumonia, and are overexpressed and purified from E. coli.

细菌铜、锌超氧化物歧化酶具有有限的同源性 与经过充分研究的真核 SOD 酶，但进行相同的 化学反应。 七个关键活性位点组氨酸是 以及金属离子，并且可能处于相同的状态 如已知结构中的定向。 必不可少的 细菌 SOD 的序列与哺乳动物的 SOD 非常不同， 许多细菌性疾病的治疗剂的可能目标。 该项目的酶来自人类病原体奈瑟氏菌 脑膜炎球菌和猪病原体胸膜肺炎放线杆菌， 并从大肠杆菌中过度表达和纯化。