HIGH RESOLUTION STRUCTURAL STUDIES OF CRYSTALLINE PILIN

结晶皮林的高分辨率结构研究

• 批准号: 6658549
• 负责人: KATRINA T FOREST
• 金额: $ 14.32万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-01 至 2003-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6658549
• 关键词: bioimaging /biomedical imaging; biomaterials; biomedical resource; proteins; radiography; structural biology

We have completed the 2.8 E structure of pilin from Neisseria gonorrhoeae, the causative agent of the widespread disease gonorrhea. Pilin forms fibrous structures on the bacterial surface which are required for colonization and establishment of disease. Ultimately, the structure will be used to guide the design of vaccines. Our goal is to understand not only the structure of the pilin monomer but the pilin fiber as well. This goal will require the solution of additional pilin crystal structures, using crystals already obtained, in other packing environments and from other species. The structure has been refined to an R-factor of 19.4% for 2s data.

我们已经完成了来自奈瑟氏球菌的pilin的2.8E结构 淋病，广泛传播的淋病的病原体。 菌毛蛋白在细菌表面形成纤维结构， 定殖和建立疾病所需的。 最终， 该结构将用于指导疫苗的设计。 我们的目标 不仅要了解菌毛蛋白单体的结构，还要了解其 还有菌毛纤维。 这个目标需要解决 额外的菌毛蛋白晶体结构，使用已经获得的晶体， 在其他包装环境和其他物种中。 结构 2s 数据的 R 因子已细化为 19.4%。