ANALYTICAL ULTRACENTRIFUGE

分析超速离心机

• 批准号: 6054971
• 负责人: MARY D BARKLEY
• 金额: $ 19.06万
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-04-01 至 2002-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6054971
• 关键词: analytical ultracentrifugation; biomedical equipment purchase

Funds are requested for purchasing an analytical ultracentrifuge, which will in turn support a new initiative in bioorganic and biophysical chemistry centered in the Chemistry Department at CWRU. This initiative features seven major users with expertise in biological macromolecules and supramolecular assemblies. Presently, there are no analytical ultracentrifuges in the state of Ohio. The requested instrument will clearly enhance research prowess and productivity on the NIH-supported projects of the major users. It will also stimulate interactions and collaborations among faculty and students in the Chemistry Department, the Medical School, and the College of Engineering at CWRU as well as nearby institutions, such as Cleveland State University and the Cleveland Clinic Foundation. Dr. Barkley (PI) is experienced in analytical ultracentrifugation, particularly in applications to protein-protein interactions. The major users represent individuals (Lee, Zagorski, Jamieson) and groups of individuals (Barkley, Surewicz, Shainoff, Qin). Dr. Lee is expert on proteolytic enzymes. Drs. Zagorski and Surewicz are expert on protein folding and misfolding. Dr. Surewicz also represents six users from the Medical School. Dr. Jamieson is expert on mucins. Dr. Shainoff is expert on blood clotting proteins and represents two users from Cleveland State University. Dr. Jun Qin is expert on protein structure determination and represents eight users from the Cleveland Clinic Foundation. The major users will bring fundamental studies of protein-protein and protein-nucleic acid interactions as well as interactions of proteins with prosthetic groups and ligands to bear on key structure-function questions in a wide range of biological systems. The synergism among their research groups spawned by-the requested instrument will greatly facilitate progress on their research projects. The requested analytical ultracentrifuge will be used to measure molecular weights as well as macromolecular association constants and stoichiometry. Also requested is a printer for hard copy output and a density meter for buoyancy factor determination. The equipment will be housed in a newly renovated laboratory of the Cleveland Center for Structural Biology (CCSB), which is the Major Analytical Instrument Facility (MAIF) in the Chemistry Department.

需要资金购买分析超速离心机，这反过来将支持以 CWRU 化学系为中心的生物有机和生物物理化学新举措。该计划由七位在生物大分子和超分子组装方面具有专业知识的主要用户组成。目前，俄亥俄州没有分析超速离心机。所需的仪器将明显提高主要用户 NIH 支持项目的研究能力和生产力。它还将促进 CWRU 化学系、医学院和工程学院以及克利夫兰州立大学和克利夫兰诊所基金会等附近机构的师生之间的互动与合作。 Barkley 博士（PI）在分析超速离心方面经验丰富，特别是在蛋白质-蛋白质相互作用的应用方面。主要用户代表个人（Lee、Zagorski、Jamieson）和个人群体（Barkley、Surewicz、Shainoff、Qin）。李博士是蛋白水解酶方面的专家。博士。 Zagorski 和 Surewicz 是蛋白质折叠和错误折叠方面的专家。 Surewicz 博士还代表医学院的六名用户。贾米森博士是粘蛋白方面的专家。 Shainoff 博士是凝血蛋白方面的专家，代表克利夫兰州立大学的两名用户。秦军博士是蛋白质结构测定方面的专家，代表克利夫兰诊所基金会的八位用户。主要用户将带来蛋白质-蛋白质和蛋白质-核酸相互作用以及蛋白质与辅基和配体相互作用的基础研究，以解决广泛的生物系统中的关键结构-功能问题。所要求的仪器所产生的研究小组之间的协同作用将极大地促进他们的研究项目的进展。所需的分析超速离心机将用于测量分子量以及大分子缔合常数和化学计量。还需要一台用于硬拷贝输出的打印机和一个用于确定浮力系数的密度计。这些设备将安置在克利夫兰结构生物学中心 (CCSB) 新装修的实验室中，该实验室是化学系的主要分析仪器设施 (MAIF)。