OPTIMA XL-I ANALYTICAL ULTRACENTRIFUGE & ACCESSORIES

OPTIMA XL-I 分析超速离心机

• 批准号: 6439969
• 负责人: ROBERT MACCOLL
• 金额: $ 29.09万
• 依托单位: WADSWORTH CENTER
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-01 至 2003-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6439969
• 关键词: analytical ultracentrifugation; biomedical equipment purchase; sedimentation velocity

DESCRIPTION (provided by applicant): The participants in this proposal require an XL-I analytical ultracentrifuge from Beckman Coulter together with an assortment of necessary accessories, to advance their research projects. The scientists involved are from the Wadsworth Center, Albany Medical College, and Albany College of Pharmacy. Currently there is no analytical ultracentrifuge in this region. The PI has extensive previous experience with the old model F analytical ultracentrifuge, as do two other participants. The instrument will be located at the Wadsworth Center in an existing core facility where the PI is core director. The PI and the associate core director will have the responsibility of becoming expert with this technology and training others in its use and applications. Since it is to be located in an existing core that has several instruments, there is considerable expertise in how to properly provide such a scientific service. A salient reason to acquire the analytical ultracentrifuge is that it will enhance the other existing instrumentation, which includes dynamic light scattering, HPLC, circular dichroism and fluorescence. The projects are mostly NIH funded, and involve research projects that the analytical ultracentrifuge is capable of handling: including the accurate determination of oligomeric states, the self-assembly properties of a protein, the study of complex formation between unlike molecules, and shape changes in molecules. Several of the projects propose to study complex interactions between unlike proteins or proteins and nucleic acids. The analytical ultracentrifuge has a number of instrumentation strategies and various powerful software protocols to solve these biologically important problems. It is a versatile and sometimes singularly appropriate technological approach to biochemical and biophysical progress.

描述（由申请人提供）：本提案的参与者要求 Beckman Coulter 的 XL-I 分析超速离心机以及 各种必要的配件，以推进他们的研究项目。这 参与的科学家来自沃兹沃斯中心、奥尔巴尼医学院和 奥尔巴尼药学院。目前国内还没有分析型超速离心机 这个地区。 PI 之前对旧型号 F 拥有丰富的经验 分析超速离心机，其他两名参与者也是如此。该仪器将 位于沃兹沃斯中心 (Wadsworth Center) 的现有核心设施中，PI 在此 核心导演。 PI和副核心董事将拥有 成为该技术专家并培训其他人的责任 它的用途和应用。由于它位于现有的核心区 拥有多种仪器，在如何正确使用方面有相当多的专业知识 提供这样科学的服务。获得分析的一个重要原因 超速离心机的优点是它将增强其他现有仪器， 其中包括动态光散射、HPLC、圆二色性和 荧光。这些项目大部分由 NIH 资助，并且涉及研究项目 分析超速离心机能够处理：包括 准确测定寡聚状态、自组装特性 蛋白质，研究不同分子之间的复杂形成以及形状 分子的变化。几个项目提议研究复杂的 不同蛋白质或蛋白质与核酸之间的相互作用。这 分析超速离心机有多种仪器策略和 各种强大的软件协议来解决这些具有重要生物学意义的问题 问题。它是一种多功能且有时非常合适的技术 生物化学和生物物理进展的方法。