CORTICAL MODULATION OF SOMATOSENSATION

躯体感觉的皮质调节

• 批准号: 6343808
• 负责人: ALDO RUSTIONI
• 金额: $ 21.6万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 1980
• 资助国家: 美国
• 起止时间: 1980-04-01 至 2002-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6343808
• 关键词: Chordata; glutamate receptor; immunocytochemistry; neural information processing; neural transmission; neuroanatomy; neuronal transport; somesthetic sensory cortex; synapses; thalamus

DESCRIPTION (Investigator's Abstract): During recent years of support from this grant the applicant has focused on immunocytochemistry for amino acids within synaptic terminals of identified descending cortical pathways as a better indicator of the neurotransmitter pool of an amino acid than the autoradiographic or immunocytochemical identification combined with anterograde tracing at the electron microscopic level, thus permitting quantification of the density of amino acids in an identified population of synaptic endings. The principal investigator found that corticofugal terminals in the thalamus, dorsal column nuclei and spinal cord were enriched with glutamate, though the density of gold particles coding for this amino acid was variable. Some terminals were also enriched with aspartate but little correlation existed between the density of particles coding for glutamate and for aspartate in the same terminal, implying that corticofugal terminals may be enriched with one amino acid but not the other. From this research it has become apparent that the cerebral cortex exerts its control by way of glutamatergic pathways even though the role of its modulation may vary in different subcortical structures. Different effects of cortical control may be mediated by different glutamate receptors especially where their control is exerted on a pool of excitatory neurons, e.g. in the ventrobasal complex (VB) of the thalamus versus a pool of inhibitory neurons, e.g. in the reticular nucleus, both parts of thalamic circuits. The main focus of the proposed research is to explore this possibility. The applicant will determine the subunit composition of glutamate receptors at the ending of corticothalamic fibers in VB and in the reticular nucleus. In addition, the applicant will also determine the co-existence of different glutamate receptors (AMPA, NMDA and metabotropic) at the same cortical synapse in VB and reticular nucleus, as well their topographic arrangement and presynaptic versus postsynaptic location.

描述（研究者的摘要）：在近年来的支持中 该赠款申请人专注于氨基酸的免疫细胞化学 在已鉴定出的皮质途径的突触终端中 比氨基酸的神经递质库更好的指标 放射自显影或免疫细胞化学识别与 在电子显微镜水平上进行顺行追踪，从而允许 量化已确定人群中氨基酸的密度 突触结局。 首席研究员发现皮质法果 丘脑，背柱核和脊髓中的末端是 富含谷氨酸，尽管金颗粒的密度编码为 该氨基酸是可变的。 一些终端也充满了 天冬氨酸，但几乎没有颗粒密度之间的相关性 在同一终端中为谷氨酸编码和天冬氨酸，这意味着 皮质法果末端可能富含一个氨基酸，但不能 其他。 从这项研究中，很明显，大脑皮质会施加 即使其作用 调节可能在不同的皮层结构中有所不同。 不同的效果 皮质控制的可能由不同的谷氨酸受体介导 特别是在将它们的控制施加在兴奋性神经元池上的情况下， 例如在丘脑的腹腔腔络合物（VB）中 抑制性神经元，例如在网状核中，丘脑的两个部分 电路。 拟议研究的主要重点是探索这种可能性。 这 申请人将确定谷氨酸受体的亚基组成 VB和网状核中皮质丘脑纤维的结束。 在 此外，申请人还将确定不同的共存 同一皮质的谷氨酸受体（AMPA，NMDA和代谢型） VB和网状核中的突触，以及它们的地形布置 以及突触前与突触后位置。