CORTICAL MODULATION OF SOMATOSENSATION

躯体感觉的皮质调节

• 批准号: 2262969
• 负责人: ALDO RUSTIONI
• 金额: $ 12.36万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 1980
• 资助国家: 美国
• 起止时间: 1980-04-01 至 1996-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2262969
• 关键词: Chordata; dorsal column; electron microscopy; glutamate receptor; glycine; immunocytochemistry; in situ hybridization; interneurons; light microscopy; neural information processing; neural transmission; neuroanatomy; neuronal transport; neurotransmitters; nitric oxide; somesthetic sensory cortex; spinal nerves; synapses

DESCRIPTION: Previous research has identified a complex pattern of sensory modulation by cortical pathways. An important area of modulation is the dorsal column nuclei. Neurons in the dorsal column nuclei may receive input from either glutamergic or aspartergic cortical synapses. This input may occur on either interneurons or relay neurons. One area of the proposed research is to determine at the EM level, using multiple label techniques, the neurochemicals contained in afferents to identified subpopulations of dorsal column nuclei neurons. This initial work will be followed by identification of amino acid receptors that are expressed by dorsal column nuclei neurons. Immunocytochemical localization will be used, as well as in situ hybridization using oligonucleotide probes. Postembedding immunogold techniques are hoped to be developed to localize receptors at the EM level. Lastly, the proposal plans to continue analysis of NO neurons and confirm that all are indeed interneurons rather than neurons with other target areas. Again, using double label methodology, colocalizaton of NO with glycine rather than GABA in dorsal column nuclei neurons will be explored.

描述：以前的研究已经确定了复杂的模式 皮质途径的感觉调节。 一个重要领域 调制是背柱核。 背侧柱中的神经元 细胞核可能会从谷氨酸或天冬酸中收到输入 皮质突触。 此输入可能发生在中间神经元或 继电器神经元。 拟议研究的一个领域是确定 EM水平使用多个标签技术，神经化学物质 包含在识别背柱亚群的传入中 细胞核神经元。 最初的工作将随后进行身份证明 由背色核表达的氨基酸受体的 神经元。 将使用免疫细胞化学定位以及 使用寡核苷酸探针的原位杂交。 邮政 希望能够开发出免疫金技术来定位受体 在EM级别。 最后，该提案计划继续分析 没有神经元，并确认所有人确实是中间神经元，而不是 具有其他目标区域的神经元。 再次使用双标签 方法论，与甘氨酸的无共钙化，而不是gaba 将探索背柱核神经元。