DESCENDING CONTROL OF SOMATOSENSORY INPUT

体感输入的下降控制

• 批准号: 3396764
• 负责人: ALDO RUSTIONI
• 金额: $ 12.38万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 1980
• 资助国家: 美国
• 起止时间: 1980-04-01 至 1993-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3396764
• 关键词: cats; dorsal column; electron microscopy; electrophysiology; gamma aminobutyrate; glutamate decarboxylase; histochemistry /cytochemistry; immunochemistry; immunocytochemistry; interneurons; neural information processing; neural transmission; neuroanatomy; neurochemistry; neurotransmitters; radionuclide double label; somesthetic sensory cortex; spinal cord; synapses; thalamic nuclei; thalamus

The proposed research deals with several aspects of the central modulation of somatosensation. One of the main goals is to identify the neurotransmitter of cortical neurons at the origin of the descending pathways to the somatosensory thalamus, the dorsal column nuclei and the spinal cord in rats. Available evidence suggests that glutamate may be involved in these neurons and we will employ an immunocytochemical approach combined with the retrograde transport of dyes to verify this. Double-labeling immunocytochemistry will be also employed to investigate whether some of the peptides known to be present in non-pyramidal cortical neurons co-localize with glutamergic neurons in layer VI. We also wish to investigate whether modulation of VB activity in cats by the cortex and other subcortical centers (e.g. raphe nuclei, periaqueductal gray, etc.) is primarily mediated via direct contacts upon intrinsic GABA - ergic neurons. Combination of immunocytochemistry against glutamic acid decarboxylase (GAD) and anterograde tracing techniques will be employed at the electron microscopical level. This part of the planned research will elucidate mechanisms presumably involved in some basic thalamic functions and will establish a solid basis for further insights in how input from different centers may affect the activity of VB neurons. As a third main goal, we'll try to answer whether GAD positive neurons in cat's VB contain peptides that are believed to be involved in some form of control of neurotransmission. The rationale for this part of the research lies mainly in the demonstration that GABA - ergic neurons, e.g. in the cortex, may express other molecular markers. Furthermore species differences in the presence of GABA - ergic neurons and co-localisation of other markers may reflect increasing elaboration and complexity of functional features of thalamic nuclei.

拟议的研究涉及中央调制的几个方面 节感。 主要目标之一是确定 在降级的起源的皮质神经元的神经递质 通向体感丘脑，背柱核和途径 大鼠的脊髓。 可用证据表明谷氨酸可能是 参与这些神经元，我们将采用免疫细胞化学方法 结合染料的逆行转运以验证这一点。 双重标记的免疫细胞化学也将用于调查 是否存在一些已知存在于非锥状皮质中的肽 神经元与第六层中的谷氨酸神经元共定位。 我们还希望调查是否通过 皮质和其他皮层下中心（例如，raphe核，周围的核 灰色等）主要是通过固有GABA的直接接触来介导的。 ERGIC神经元。 免疫细胞化学对谷氨酸的结合 脱羧酶（GAD）和顺行示踪技术将采用 电子显微镜水平。 计划研究的这一部分将 阐明大概参与了一些基本丘脑功能的机制 并将为进一步的见解建立扎实的基础 不同的中心可能会影响VB神经元的活性。 作为第三个主要目标，我们将尝试回答GAD阳性神经元是否 猫的VB包含据信以某种形式涉及的肽 神经传递的控制。 这部分研究的基本原理 主要在于gaba -ergic神经元的演示，例如在 皮层可以表达其他分子标记。 此外，物种 GABA-埃及神经元的存在和共定位的差异 其他标记可能反映出越来越大的阐述和复杂性 丘脑核的功能特征。