DESCENDING CONTROL OF SOMATOSENSORY INPUT

体感输入的下降控制

• 批准号: 3396762
• 负责人: ALDO RUSTIONI
• 金额: $ 11.12万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 1980
• 资助国家: 美国
• 起止时间: 1980-04-01 至 1986-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3396762
• 关键词: aminoacid transport; aspartate; autoradiography; brain cell; electron microscopy; electrophysiology; glutamates; histochemistry /cytochemistry; immunochemistry; interneurons; neural information processing; neuroanatomy; neurophysiology; single cell analysis; synapses

The proposed research will deal with three aspects of descending control of somatosensory input and it involves several advanced neuroanatomical techniques. In one project we will pursue a research on the organization of descending cortical input to the cuneate nucleus of cats by intraaxonal staining with horseradish peroxidase. Light- and electro microscopical study of individual cortical fibers and their terminals from different functional areas will be carried out to obtain evidence relevant to the mechanisms by which the cerebral cortex modulates information processing by cuneate neurons. Another part of the proposed research is aimed at verifying the hypothesis that inerneurons in the ventrobasal complex of cats are GABA-ergic and to explore, by immunocytochemical and autoradiographic techniques, synaptic relationships within the somatosensory thalamic relay. A third project will employ the selective retrograde transport of amino acids to investigate whether cortical neurons to the ventrobasal complex of cats may use glutamate or aspartate as neurotransmitters and to verify the assumption that selective retrograde transport is a reliable experimental approach for the identification of the neurotransmitter used by a given pathway.

拟议的研究将处理降级控制的三个方面 体感输入，它涉及几个高级神经解剖学 技术。 在一个项目中，我们将对组织进行研究 通过司法部内降层皮质输入到猫的cuneate核的 用辣根过氧化物酶染色。 光和电学显微镜 研究各个皮质纤维及其终端的研究 功能区将进行以获取与 大脑皮层通过调节信息处理的机制 Cuneate神经元。 拟议研究的另一部分是针对 验证以下假设： 猫是加巴的，可以通过免疫细胞化学和 放射自显影技术，内部的突触关系 体感丘脑继电器。 第三个项目将采用选择性 氨基酸的逆行运输以研究皮质神经元是否 猫的腹腔腔综合体可以使用谷氨酸或天冬氨酸 神经递质并验证选择性逆行的假设 运输是鉴定的可靠实验方法 给定途径使用的神经递质。