FLOW IN THICK TISSUES PROBED BY DIFFUSING LIGHT

通过漫射光探测厚组织中的血流

• 批准号: 6389630
• 负责人: ARJUN G. YODH
• 金额: $ 23.78万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-04-01 至 2003-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6389630
• 关键词: athymic mouse; bioimaging /biomedical imaging; biological models; biomedical equipment development; blood flow measurement; clinical research; diagnosis design /evaluation; human subject; laboratory mouse; laboratory rat; light scattering; model design /development; nephelometry; noninvasive diagnosis; optics; phantom model; spectrometry

DESCRIPTION (Adapted from Applicant's Abstract): The concentration, oxygenation, and flow characteristics of blood cells in the microvasculature profoundly affect nourishment and thermal regulation of surrounding tissues. If these blood variables become abnormal, then tissue health may be threatened. Measurement of these basic blood parameters can reveal information about severe burns, reconstructive surgeries, peripheral vascular disease (PVD), brain metabolism and functional heterogeneity, internal bleeding, tumor vasculature, lymph flow, and ischemia. The goal of this competing continuation grant is to improve, validate and apply diffuse optical spectroscopy for the investigation of blood dynamics in deep tissues. New instrumentation has been built that combines for the first time photon correlation technology for studies of tissue internal motions such as blood flow, with diffuse photon density wave spectroscopic tools for studies of tissue absorption and scattering. The new instrument makes possible noninvasive separation of blood flow, blood oxygenation scattering, and blood volume tissue responses. While the experimental results to date are useful and reveal promise for eventual research and clinical application, the applicants have identified several target areas wherein continued progress is critical to transfer this methodology from the developmental stage toward clinical applications. The proposed research has three interrelated components. A theoretical component develops better models specific to physiological problems the applicants plan to investigate in murine tumors, rat brain, and on human limbs. A second component validates the instruments and analysis in well defined tissue phantoms and physiological models for which the biological response is either well understood and/or is measured with another experimental technique. The last component investigates how transferability of the composite optical diagnostics can be used in: (1) State-of-the-art animal studies of tumor necrosis factor and cerebral metabolic rate, and (2) Ongoing clinical studies of peripheral vascular response.

描述（根据申请人的摘要改编）：集中度， 微脉管系统中血细胞的氧合和流量特征 深刻影响周围组织的滋养和热调节。如果 这些血液变量变得异常，然后可能会威胁组织健康。 这些基本血液参数的测量可以揭示有关严重的信息 烧伤，重建手术，周围血管疾病（PVD），大脑 代谢和功能异质性，内部出血，肿瘤脉管系统， 淋巴流量和缺血。这项竞争延续赠款的目标是 改进，验证并应用弥散光谱进行研究 深层组织中的血液动力学。已经建立了新的仪器 结合第一次用于组织研究的光子相关技术 内部运动，例如血流，具有分散光子密度波 用于研究组织吸收和散射的光谱工具。新的 仪器使血流无创分离，血液 氧合散射和血容量组织反应。而 迄今为止的实验结果很有用，并揭示了最终的希望 研究和临床应用，申请人已经确定了几个 持续进展至关重要的目标领域对于转移这一点至关重要 从发育阶段到临床应用的方法。这 拟议的研究具有三个相互关联的组件。理论组成部分 开发针对申请人计划的生理问题的更好模型 在鼠肿瘤，大鼠脑和人类四肢中进行研究。第二 组件验证了定义明确的组织中的仪器和分析 幻象和生物学反应的生理模型是 通过另一种实验技术来理解和/或测量。这 最后一个组件研究了复合光学的可传递性 诊断可用于以下诊断：（1）最先进的肿瘤动物研究 坏死因子和脑代谢率和（2）正在进行的临床研究 外周血管反应。

项目成果

Diffuse optical measurement of hemoglobin and cerebral blood flow in rat brain during hypercapnia, hypoxia and cardiac arrest.

高碳酸血症、缺氧和心脏骤停期间大鼠脑血红蛋白和脑血流量的漫反射光学测量。

• DOI: 10.1007/978-1-4615-0205-0_48
• 发表时间: 2003
• 期刊: Advances in experimental medicine and biology
• 作者: Culver,JosephP;Durduran,Turgut;Cheung,Cecil;Furuya,Daisuke;Greenberg,JoelH;Yodh,AG
• 通讯作者: Yodh,AG