FLOW IN THICK TISSUES PROBED BY DIFFUSING LIGHT

通过漫射光探测厚组织中的血流

• 批准号: 6128002
• 负责人: ARJUN G. YODH
• 金额: $ 25.11万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-04-01 至 2003-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6128002
• 关键词: athymic mouse; bioimaging /biomedical imaging; biological models; biomedical equipment development; blood flow measurement; clinical research; diagnosis design /evaluation; human subject; laboratory mouse; laboratory rat; light scattering; model design /development; nephelometry; noninvasive diagnosis; optics; phantom model; spectrometry

DESCRIPTION (Adapted from Applicant's Abstract): The concentration, oxygenation, and flow characteristics of blood cells in the microvasculature profoundly affect nourishment and thermal regulation of surrounding tissues. If these blood variables become abnormal, then tissue health may be threatened. Measurement of these basic blood parameters can reveal information about severe burns, reconstructive surgeries, peripheral vascular disease (PVD), brain metabolism and functional heterogeneity, internal bleeding, tumor vasculature, lymph flow, and ischemia. The goal of this competing continuation grant is to improve, validate and apply diffuse optical spectroscopy for the investigation of blood dynamics in deep tissues. New instrumentation has been built that combines for the first time photon correlation technology for studies of tissue internal motions such as blood flow, with diffuse photon density wave spectroscopic tools for studies of tissue absorption and scattering. The new instrument makes possible noninvasive separation of blood flow, blood oxygenation scattering, and blood volume tissue responses. While the experimental results to date are useful and reveal promise for eventual research and clinical application, the applicants have identified several target areas wherein continued progress is critical to transfer this methodology from the developmental stage toward clinical applications. The proposed research has three interrelated components. A theoretical component develops better models specific to physiological problems the applicants plan to investigate in murine tumors, rat brain, and on human limbs. A second component validates the instruments and analysis in well defined tissue phantoms and physiological models for which the biological response is either well understood and/or is measured with another experimental technique. The last component investigates how transferability of the composite optical diagnostics can be used in: (1) State-of-the-art animal studies of tumor necrosis factor and cerebral metabolic rate, and (2) Ongoing clinical studies of peripheral vascular response.

描述（改编自申请人的摘要）：浓度， 微血管中血细胞的氧合和流动特性 深刻影响周围组织的营养和热调节。如果 这些血液变量变得异常，那么组织健康可能会受到威胁。 这些基本血液参数的测量可以揭示严重程度的信息 烧伤、重建手术、周围血管疾病 (PVD)、脑部 代谢和功能异质性、内出血、肿瘤血管系统、 淋巴液流动和缺血。这项竞争性持续拨款的目标是 改进、验证和应用漫反射光谱进行研究 深部组织的血液动力学。新的仪器已经建成 首次结合光子相关技术进行组织研究 内部运动，例如血流，具有漫射光子密度波 用于研究组织吸收和散射的光谱工具。新的 仪器使血流、血液的无创分离成为可能 氧合散射和血容量组织反应。虽然 迄今为止的实验结果是有用的，并揭示了最终的希望 研究和临床应用，申请人已经确定了几种 持续取得进展对于转移这一目标至关重要的目标领域 从开发阶段到临床应用的方法论。这 拟议的研究由三个相互关联的组成部分组成。理论部分 针对申请人计划的生理问题开发更好的模型 研究小鼠肿瘤、大鼠大脑和人类四肢。一秒钟 组件在明确的组织中验证仪器和分析 幻象和生理模型，其生物反应是 很好理解和/或用另一种实验技术测量。这 最后一个组件研究了复合光学材料的可转移性 诊断可用于： (1) 最先进的肿瘤动物研究 坏死因子和脑代谢率，以及（2）正在进行的临床研究 周围血管反应。