Regulation of Juvenile Hormone levels in mosquitos

蚊子保幼激素水平的调节

• 批准号: 6382658
• 负责人: Fernando Gabriel. Noriega
• 金额: $ 29.79万
• 依托单位: UNIVERSITY OF ARIZONA
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-07-01 至 2004-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6382658
• 关键词: Aedes; biological signal transduction; ganglions; gas chromatography mass spectrometry; gene expression; hormone biosynthesis; hormone regulation /control mechanism; juvenile hormones; microarray technology; neuroendocrine system; neuropeptides; neuroregulation; northern blottings; nutrition; nutrition related tag; radiochemistry; radioimmunoassay; trypsin

DESCRIPTION (provided by the applicant): Mosquito-transmitted parasitic diseases are among the major causes of mortality and morbidity in the world. Recent dramatic increases in the incidence of mosquito-borne diseases underscore the need for new approaches to insect control based on mosquito specific agents. The discovery of such mosquito-specific agents depends on basicresearch on the biology of mosquitoes. However, there is a serious deficiency in our understanding of nearly all aspects of mosquito biology. Juvenile Hormone (JH) is amajor hormonal regulator in insects. In adult female mosquito JH signals that ecdysis to the adult stage is complete and reproductive processes should begin, inducing simultaneous changes in the expression of many genes in ovarian, fat body and midgut tissues. We have shown that JH plays an essential role in the transcriptionalactivation of the early trypsin gene in the Aedes aegypti midgut. The early trypsin is aremarkable"molecular marker," its expression is dramatically modified after feeding, and it is affected by both hormones and nutrients. We propose to use the expression of theearly trypsin gene as a paradigm for the nutritional regulation of JET levels. The ultimate goal of this project is to understand how changes in the nutritional status are assessed, and this information is transduced to coordinate post-feeding physiology. In this proposal, we are focusing on thenutritional regulation of JH levels. Our hypothesis is that the midgut can detect the nutritional status after feeding, and regulates the JR levels by affecting the activity of the corpora allata (CA). This regulation could be mediated directly or indirectly bymidgut peptides synthesized by the endocrine cells and released into the hemolymph. We have established an in vitro radiochemical assay to measure the synthesis of JH by the CA, using this assay we have shown that midgut peptide extracts stimulate thesynthesis of JET by the CA, and also that an Aedes aegyptiallatotropin stimulatesthesynthesis of JET by the CA. In order to define how JR levels are regulated after a blood meal, the following specific aims are proposed: (1) To investigate the role of the Aedes aegypti allatotropin and allatostatins on the regulation of CA activity in vitro, (2) To identify identify the peptide(s) responsible for the midgut allato-regulatory activity and (3) To investigate the physiological role of allato-regulatory peptides "in vivo."

描述（申请人提供）：蚊子传播的寄生虫 疾病是世界上死亡率和发病率的主要原因之一。 蚊子传播疾病的发生率最近发生了巨大增加 强调了基于蚊子的新方法进行昆虫控制的需求 具体代理。这种蚊子特异性药物的发现取决于 蚊子生物学的基础研究。但是，有一个严重的 我们对蚊子生物学几乎所有方面的理解不足。 青少年激素（JH）是昆虫中的Amajor激素调节剂。在成年女性中 Mosquito JH信号表明，对成人阶段的逐渐分析已完成，并且 生殖过程应开始，引起同时改变 许多基因在卵巢，脂肪体和中肠组织中的表达。我们已经显示了 JH在早期的转录激活中起着至关重要的作用 伊德斯埃及埃及中肠中的胰蛋白酶基因。早期胰蛋白酶是 表达式的表达方式大大修饰 进食，并受激素和营养的影响。我们建议使用 肌胰蛋白酶基因作为营养的范例的表达 喷气水平的调节。该项目的最终目标是了解 如何评估营养状况的变化，此信息是 转导至协调后喂养生理学。在这个建议中，我们是 重点关注JH水平的静脉调节。我们的假设是 中肠可以在进食后检测营养状况，并调节JR 通过影响Corpora allata（CA）的活动来级别。这个法规 可以直接或间接地介导由 内分泌细胞并释放到血淋巴中。我们已经建立了一个 使用此功 测定我们已经表明，中肠肽提取物刺激射流的菌株 由CA，以及伊德岛的埃及latotropin刺激性刺激性 由CA的喷气机。为了定义血液后如何调节JR水平 餐，提出了以下具体目标：（1）调查的作用 CA的调节中的埃及伊蚊Allatotropin和allatostatins 在体外活动，（2）确定负责的肽 Midgut Allato调节活性和（3）研究生理作用 Allato调节性肽“体内”。