INFORMATICS CENTER FOR MOUSE NEUROGENETICS

小鼠神经遗传学信息中心

• 批准号: 6190398
• 负责人: ROBERT W. WILLIAMS
• 金额: $ 127.04万
• 依托单位: UNIVERSITY OF TENNESSEE HEALTH SCI CTR
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-28 至 2005-08-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6190398
• 关键词: Internet; bioimaging /biomedical imaging; brain imaging /visualization /scanning; brain mapping; computer system design /evaluation; educational resource design /development; genetic mapping; laboratory mouse; molecular biology information system; morphometry; neurogenetics; quantitative trait loci

DESCRIPTION (Applicant's Abstract): The purpose of this Neuroinformatics project is to develop and exploit a suite of image databases, motorized Internet microscopes, and software to study the genetic basis of structural variation of the mouse CNS. Resources are open to the research community through an integrated web interface at <nervenet.org>. The focus of this project is to provide a collaborative research environment for mapping quantitative trait loci (QTLs). These genes are responsible for the extraordinary variation in CNS structure among mice and humans. QTL analysis is a burgeoning field that tackles complex biological traits modulated by many genes. We will develop four significant new resources and technologies: (1) The Mouse Brain Library (MBL) consists of a huge, well-organized library of brain sections suitable for morphometric investigation. Thousands of images can be rapidly searched, sorted, and downloaded at a resolution of 5 microns per pixel using an intuitive and powerful web interface. (2) The Internet Microscope System (iScope) captures and displays extremely detailed movies -z-axis image stacks-suitable for sophisticated stereological study of all brains in the MBL. The iScope includes robotic slide handlers controlled over the web 24 hours a day, 7 days a week (3) The NeuroCartographer Project will develop a suite of software tools and 3D models of hundreds of neuroanatomical structures that will enable researchers to reconstruct and digitally dissect; material in the MBL. (4) The Neurogenetics Tool Box (NTB) comprises a set of gene mapping programs that will enable neuroscientists to rapidly identify and evaluate QTLs responsible for the astonishing variation in CNS architecture. The NTB will include genotypes from an unusually large advanced intercross designed to map loci with sufficient precision to enable a candidate gene approach to cloning QTLs. Achieving the aims of these four projects will catalyze a new era in the structural analysis of the adult mammalian nervous system and will lead to a large number of novel lines of research on the development, aging, and pathology of the human brain.

描述（申请人的摘要）：此目的 神经信息学项目是开发和利用一套图像数据库， 电动互联网显微镜和研究遗传基础的软件 小鼠中枢神经系统的结构变异。资源对研究开放 通过 <nervenet.org> 上的集成 Web 界面建立社区。重点是 该项目旨在为测绘提供协作研究环境 数量性状基因座（QTL）。这些基因负责 小鼠和人类的中枢神经系统结构存在巨大差异。 QTL分析是 这是一个新兴领域，致力于解决受许多因素调节的复杂生物特征 基因。我们将开发四项重要的新资源和新技术：（1） 小鼠大脑图书馆（MBL）由一个庞大、组织良好的大脑图书馆组成 适合形态测量研究的切片。可以存储数千张图像 以每像素 5 微米的分辨率快速搜索、排序和下载 使用直观且功能强大的网络界面。 (2) 互联网显微镜 系统 (iScope) 捕获并显示极其详细的电影 -z 轴图像 堆栈 - 适用于 MBL 中所有大脑的复杂体视学研究。 iScope 包括通过网络 24 小时控制的机器人载玻片处理程序 每天，每周 7 天 (3) NeuroCartographer 项目将开发一套 软件工具和数百个神经解剖结构的 3D 模型 将使研究人员能够重建和数字化解剖；材料在 MBL。 (4) 神经遗传学工具箱（NTB）包含一组基因图谱 使神经科学家能够快速识别和评估 QTL 的程序 造成中枢神经系统结构惊人变化的原因。 NTB 将 包括来自异常大的先进杂交的基因型，旨在绘制地图 具有足够精度的位点以实现候选基因克隆方法 QTL。实现这四个项目的目标将催生一个新时代 成年哺乳动物神经系统的结构分析，将导致 大量关于发育、老化和 人脑的病理学。