EVALUATION OF PULSE WIDTH DEPENDENCE OF MULTI PHOTON PHOTO DAMAGE

多光子照片损伤的脉冲宽度依赖性评估

• 批准号: 6349417
• 负责人: JENNIFER A NICHOLS
• 金额: $ 0.78万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-01 至 2001-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6349417
• 关键词: animal tissue; bioengineering /biomedical engineering; biological products; biomedical equipment development; biomedical resource; lasers; neoplasm /cancer; technology /technique

Within the last year, cytochrome c release from the mitochondria has been identified as a very early event during apoptosis, or programmed cell death. Furthermore, a loss of mitochondrial membrane potential is observed during the mitochondrial permeability transition, which results from oxidative stress injuries. This has led us to inquire whether multiphoton imaging of mitochondrial NADH may provide a technique to observe mitochondrial dysfunction without the use of exogenous mitochondrial probes. Our recent efforts have been directed at establishing protocols to induce apoptosis and/or mPT in rodent and/or human cancer cell lines. Future experiments will seek to correlate changes in mitochondrial autofluorescence with mitochondrial morphology and membrane potential as observed with fluorescent probes.

去年，细胞色素 c 从线粒体中释放 已被确定为细胞凋亡过程中的一个非常早期的事件，或 程序性细胞死亡。 此外，线粒体膜的丧失 在线粒体通透性过程中观察到电位 转变，这是由氧化应激损伤引起的。 这有 引导我们探究线粒体 NADH 的多光子成像是否 可能提供一种观察线粒体功能障碍的技术，而无需 使用外源线粒体探针。 我们最近的努力 致力于建立诱导细胞凋亡和/或 mPT 的方案 在啮齿动物和/或人类癌细胞系中。 未来的实验将 寻求将线粒体自发荧光的变化与 线粒体形态和膜电位观察 荧光探针。