ACTION SPECTRUM OF DNA SYNTHESIS INHIBITION USING FEMTOSECOND ILLUMINATION

使用飞秒照明抑制 DNA 合成的作用谱

• 批准号: 6121859
• 负责人: JENNIFER A NICHOLS
• 金额: $ 13.66万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-30 至 1999-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6121859
• 关键词: bioengineering /biomedical engineering; biological products; biomedical equipment development; biomedical resource; biotechnology; lasers; technology /technique

We have produced an action spectrum of photodamage using femtosecond illumination from 700 to 900 nm by monitoring the onset of DNA synthesis inhibition in cultured HeLa cells without added dyes. Doses (Int) are defined as the nth power of the peak irradiance at the sample multiplied by the total dwell time of the focal volume in a cell. (The irradiance, I, is in photons/s((m2, and n is the number of photons required for a single excitation event, or the excitation order.) Since we have not yet determined the excitation order of damage at all wavelengths, this action spectrum is expressed with n = 2, defining two-photon events. The measured damage thresholds are orders of magnitude above typical imaging doses.

我们使用以下方法制作了光损伤的作用谱 通过监测 700 至 900 nm 的飞秒照明 不添加染料的培养 HeLa 细胞中 DNA 合成抑制。 剂量 (Int) 定义为峰值辐照度的 n 次方 样本乘以焦点体积的总停留时间 细胞。 （辐照度 I 的单位是光子/秒（（m2，n 是光子数 单个激发事件或激发所需的光子 阶。）由于我们还没有确定激励阶 所有波长的损伤，该作用光谱用 n = 表示 2、定义双光子事件。 测量的损伤阈值是 比典型成像剂量高出几个数量级。