ACTION SPECTRUM OF DNA SYNTHESIS INHIBITION USING FEMTOSECOND ILLUMINATION

使用飞秒照明抑制 DNA 合成的作用谱

• 批准号: 6121859
• 负责人: JENNIFER A NICHOLS
• 金额: $ 13.66万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-30 至 1999-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6121859
• 关键词: bioengineering /biomedical engineering; biological products; biomedical equipment development; biomedical resource; biotechnology; lasers; technology /technique

We have produced an action spectrum of photodamage using femtosecond illumination from 700 to 900 nm by monitoring the onset of DNA synthesis inhibition in cultured HeLa cells without added dyes. Doses (Int) are defined as the nth power of the peak irradiance at the sample multiplied by the total dwell time of the focal volume in a cell. (The irradiance, I, is in photons/s((m2, and n is the number of photons required for a single excitation event, or the excitation order.) Since we have not yet determined the excitation order of damage at all wavelengths, this action spectrum is expressed with n = 2, defining two-photon events. The measured damage thresholds are orders of magnitude above typical imaging doses.

我们使用 通过监测开始的飞秒照明从700到900 nm 无添加染料的培养的HeLa细胞中的DNA合成抑制。 剂量（int）定义为峰辐照度的第n个功率 样品乘以焦点的总停留时间 细胞。 （辐照度I为光子/s（（M2，N是 一次激发事件或激发所需的光子 订单。）由于我们尚未确定 在所有波长的损害中，此动作谱均以n =表示 2，定义两光子事件。 测量的伤害阈值是 高于典型成像剂量的数量级。