ANALYTICAL ULTRACENTRIFUGE FOR MACROMOLECULAR STUDIES

用于大分子研究的分析超速离心机

• 批准号: 6292022
• 负责人: Cynthia Vianne Stauffacher
• 金额: $ 27.06万
• 依托单位: PURDUE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-03-01 至 2002-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6292022
• 关键词: analytical ultracentrifugation; biomedical equipment purchase; intermolecular interaction; macromolecule

The properties and capabilities are described of an analytical ultracentrifuge, Beckman Optima XL-I, with both interference and absorption optics, that will be used by a group of Purdue research scientists to investigate the interactions between macromolecules in biologically important systems. The acquisition of this instrument is part of a plan to assemble an array of instruments in a center for biophysical studies of macromolecules. This array of instruments currently includes a surface plasmon resonance instrument, an isothermal calorimeter, and a ten-year old Beckman Model XL-A analytical ultracentrifuge. Future development of this center targets the addition of state-of-the-art fluorescence, FTIR and Raman spectrometers, plus a stopped flow rapid reaction instrument. This proposal for the XL-I analytical ultracentrifuge is submitted by a group of six investigators involved in studies of protein-protein, protein-nucleic acid, protein-ligand and protein- membrane interactions. These investigators have expertise in high resolution crystallographic structural studies as well as in biochemistry, biophysics and molecular genetics, and are interested in complementing their structural results with the biophysical measurements that can illuminate the details of assembly of large complexes, changes in interaction states with ligand substrates or effectors or with modifications that mimic cellular signaling states. Investigations of stability of large complexes and search for conditions of monodisperse preparations of membrane proteins are also proposed, as an aid to the crystallization of these difficult systems. Initial studies with the Beckman XL-A have demonstrated the usefulness of analytical ultracentrifugation in a wide variety of systems including: the binding affinity of membrane proteins for their soluble counterparts, assembly of animal viruses, signal transduction protein interactions, interactions between toxins and their cellular receptors as well as the assembly of large protein complexes for transmembrane transport. The XL-I will extend the range of studies into those involving weaker interactions as well as to studies where the optical characteristics of the samples are inappropriate for the absorption optics of the XL-A and will compliment and extend the information obtained by both structural and biophysical methods at Purdue.

描述了具有干扰和吸收光学元件的分析性超级离心XL-I的特性和能力，这些XL-I将被一组Purdue Research Scientists使用，以研究生物学重要系统中的大分子之间的相互作用。该仪器的获取是在大分子生物物理研究中心组装一系列仪器的计划的一部分。目前，这类仪器包括表面等离子体共振仪器，等温量热仪和十年历史的贝克曼型XL-A分析性超级分析。该中心的未来开发目标是添加最先进的荧光，FTIR和拉曼光谱仪，以及停止的流动快速反应仪器。 XL-1分析超级离心液的这一建议是由参与蛋白质蛋白质，蛋白质核酸，蛋白质 - 配体和蛋白质膜相互作用研究的六名研究人员提出的。这些研究者在高分辨率晶体结构研究以及生物化学，生物物理学和分子遗传学方面都有专业知识，并有兴趣通过生物物理测量值补充其结构性结果，这些测量可以阐明大型复合物的组装细节，与配件式效应或效应态度的变化状态的变化，以拟态的细胞效应。还提出了对大型复合物的稳定性和寻找膜蛋白单分散制剂的条件的研究，以帮助这些困难系统的结晶。对Beckman XL-A的初步研究证明了在多种系统中分析超离心的有用性，包括：膜蛋白对其可溶性对应物的结合亲和力，动物病毒的组装，信号转导蛋白相互作用，毒素及其蜂窝受体之间的相互作用以及大型蛋白质复合物之间的相互作用。 XL-I将把研究范围扩展到涉及较弱相互作用的研究范围以及样品的光学特征不适合XL-A的吸收光学的研究，并将补充和扩展Purdue中结构和生物物理方法获得的信息。