MODELING OF NEUROTRANSMITTER GATED CHANNELS, RECEPTORS, PROTEINS: STRUC & FUNCT

神经递质门控通道、受体、蛋白质的建模：STRUC

• 批准号: 6295167
• 负责人: MICHAEL CASCIO
• 金额: $ 1.19万
• 依托单位: MELLON PITTS CORPORATION (MPC CORP)
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-30 至 1999-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6295167
• 关键词: Chordata; Invertebrata; Mammalia; aging; animal tissue; biomedical resource; carbohydrates; cognition; computers; endocrine gland /system; enzymes; immunology; inflammation; minerals; nervous system

The overall goal of this project is to examine, at a molecular level, the correlation between structure and function for several neurotransmitter-gated channels, receptors, and related proteins. Currently, the main focus of the laboratory is the examination of the secondary structure, topology, and functional role of domains and specific residues of the homomeric recombinant human $alph$1 glycine receptor overexpressed in a baculovirus expression system. This inhibitory gated anionic channel is a member of the ligand-gated superfamily, which include the homologous nicotinic acetylcholine receptor, the 5-HT3 serotonin receptor, and the GABA receptor, all of which act in rapid mediation of signal transduction at the synapse. These investigations may provide insight into the general conserved mechanism used in channel design. Analogous studies concerning the NMDA type glumatmate receptors and the gonadotropin releasing hormones (GnRH) receptor will also be conducted. These receptors, too, are members of large families, and may their structural featur may be paradigmatic; the NMDA receptor is a member of the excitatory glutamate receptor family which act in synaptic transmission and excitotoxicity, and the GnRH receptor is a member of the large G protein coupled receptors and is a pivotal role in regulating coordination of the neural and endocrine systems. In addition, crystalliszation trails of EP 24.15 a zinc metalloendopeptidase which degrades GnRH, are also underway.

该项目的总体目标是在分子水平上检查 水平，几个结构和功能之间的相关性 神经递质门控通道、受体和相关蛋白质。 目前，实验室的主要工作是检测 域的二级结构、拓扑和功能作用 同聚重组人 $alph$1 甘氨酸的特定残基 杆状病毒表达系统中过度表达的受体。 这 抑制性门控阴离子通道是配体门控通道的成员 超家族，包括同源烟碱乙酰胆碱 受体、5-HT3 血清素受体和 GABA 受体 它在突触信号转导中发挥快速调节作用。 这些研究可以深入了解一般保守的 渠道设计中使用的机制。 类似的研究涉及 NMDA 型谷氨酸受体和促性腺激素释放激素 (GnRH) 受体也将进行。 这些受体也 大家庭的成员，他们的结构特征可能是 范式； NMDA 受体是兴奋性受体的成员 谷氨酸受体家族，在突触传递中起作用 兴奋性毒性，GnRH 受体是大 G 的成员 蛋白偶联受体，在调节中起关键作用 神经系统和内分泌系统的协调。 此外， EP 24.15 锌金属肽链内切酶的结晶轨迹 降解 GnRH 的过程也在进行中。