GENETIC LINKAGE MAPPING OF CHROMOSOME 13

13 号染色体的遗传连锁图谱

• 批准号: 6221057
• 负责人: ROBERT E FERRELL
• 金额: $ 0.13万
• 依托单位: MELLON PITTS CORPORATION (MPC CORP)
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-08-01 至 2000-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6221057
• 关键词: Chordata; Invertebrata; Mammalia; animal tissue; biomedical resource; carbohydrates; enzymes; growth /development; minerals; nervous system

The research plan involves examining the different families of calmodulin-dependent enzymes to determine: (1) structural or functional motifs (2) hydrophilicity/hydrophobicity index of putative calmodulin-binding regions; (3) the position of phosphorylation sites or pseudosubstrate domains or the homologous regions relative to the calmodulin-binding domain; (4) searching database for sequences similar to regions associated with calmodulin binding; (5) secondary structure prediction in the vicinity of the calmodulin (CAM) binding site; (6) internal repeated sequences via dot plot; (6) examination of local multiple alignments by dynamic programming procedures. Enzyme sequences to be investigated will include the entire spectrum of CAM target proteins. The spectrum includes: Class 1 target proteins that bind to CAM without associated Ca2+ and have little affinity for Ca2+-CAM; Class 2 proteins that bind CAM strongly in the absence of Ca2+ and are positively activated by Ca2+ ; and Class 3 proteins that have high affinity for the CAM-Ca2+ and weak or no affinity for Class 2 are macrophage NO synthases, lung cyclic 3', 5'-nucleotide phosphodiesterase, bordetella pertussis adenylate cyclase, and glycogen phosphorylase b kinase; and class 3 containing the remaining enzymes and proteins such as calcineurin, myosin light chain kinase, caldesmon, adenylate cyclases, fodrin, IP3 kinase, marcks protein, adducins, protein kinases, Ca2+ ATPase pumps and related proteins.

该研究计划涉及检查不同的家族 钙调蛋白依赖性酶确定：（1）结构或 功能基序 (2) 假定的亲水性/疏水性指数 钙调蛋白结合区； (3)磷酸化位点的位置 或假底物结构域或相对于的同源区域 钙调蛋白结合域； (4) 搜索数据库中的序列 与钙调蛋白结合相关的区域相似； (5) 二级 钙调蛋白 (CAM) 结合附近的结构预测 地点; (6) 通过点图表示内部重复序列； (6)检查 通过动态编程过程进行局部多重比对。 酶 待研究的序列将包括 CAM 的整个谱 目标蛋白质。 该谱包括： 1 类目标蛋白 与 CAM 结合，无需结合 Ca2+，且对 CAM 的亲和力很小 Ca2+-CAM； 2 类蛋白在缺乏 CAM 的情况下强烈结合 CAM Ca2+ 和 被 Ca2+ 积极激活；和 3 类蛋白质 对 CAM-Ca2+ 具有高亲和力，对 Class 具有弱或无亲和力 2 是巨噬细胞 NO 合酶，肺环状 3', 5'-核苷酸 磷酸二酯酶、百日咳博德特氏菌腺苷酸环化酶，和 糖原磷酸化酶b激酶；和第 3 类包含剩余的 酶和蛋白质，例如钙调神经磷酸酶、肌球蛋白轻链激酶、 caldesmon、腺苷酸环化酶、fodrin、IP3 激酶、marcks 蛋白、 内收蛋白、蛋白激酶、Ca2+ ATP酶泵和相关蛋白。