CORE--BONE MORPHOMETRY AND MOLECULAR CYTOIMAGING

核心——骨形态学和分子细胞成像

• 批准号: 6316958
• 负责人: ROBERT Stewart WEINSTEIN
• 金额: $ 19.64万
• 依托单位: UNIV OF ARKANSAS FOR MED SCIS
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-06-01 至 2001-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6316958
• 关键词: animal tissue; bioimaging /biomedical imaging; biomedical facility; bone density; bone marrow; histology; human tissue; in situ hybridization; information systems; photon absorptiometry; polymerase chain reaction; tissue /cell culture

The purpose of the Bone Morphometry and Molecular Cytoimaging Core is to consolidate key personnel and equipment to provide a centralized resource facility to enhance collaborative and multidisciplinary investigations into the cellular and molecular mechanisms of osteoporosis. The Core will perform three different procedures in a high-quality, reliable and cost- effective manner: bone densitometry, bone histomorphometry and histostaining of bone tissue and bone marrow cells combined with in situ hybridization or in situ use of the reverse transcriptase-polymerase chain reaction (RT-PCR). The measurement of longitudinal changes in murine bone mineral density by dual-energy X-ray absorptiometry (DXA or DEXA), a technique we have developed for this Program, is used to determine the differences in bone density in the senescence accelerated mouse (SAM) and its hybrid progeny, the changes in bone density with age, gonadectomy and treatment in thee mice and other strains and the optimal time for ex-vivo cell cultures and histomorphometric analysis of undecalcified bone specimens. The noninvasive and precise nature of DEXA supersedes more traditional methods for the estimation of murine bone magnitude such as ashing or cortical width morphometry. This core will also obtain, fix, embed, section, stain and quantify the bone histomorphometric features of the axial and appendicular skeleton of young and old mice for the needs of the various projects. In preliminary work leading to this application, it has become evident that the concurrent performance on adjacent bone sections of RT-PCR for specific messages and histostaining with cell markers such as tartrate resistant acid phosphatase (TRAP) for osteoclasts, nonspecific esterase (NSE) for macrophages or alkaline phosphatase (ALP) for osteoblasts combined with tetracycline-labeled bone histomorphometry is required to characterize distinct subsets of bone and bone marrow cells, their gene expression patterns and their activity. This core has done over 400 murine DEXA measurements in-vivo over the past six months (sharing use of a clinical densitometer on weekends from 8 A.M. to 10 P.M. or on weekdays after 5 P.M.) and has processed 85 human bone biopsies and over 60 murine specimens for histomorphometric examinations in the past year. The bone laboratory is a CAP accredited facility with the ability to safely archive and store tissue and data. Bone densitometry and histomorphometric results will be appended to a relational database for prompt return to the principal investigators of each project.

骨形态计量学和分子细胞成像核心的目的是 合并关键人员和设备以提供集中资源 设施以增强协作和多学科调查 进入骨质疏松症的细胞和分子机制。 核心意愿 在高质量，可靠且成本上执行三种不同的程序 有效的方式：骨密度测定法，骨骼组态法和 骨组织和骨髓细胞的组织稳定与原位结合 杂交或原位使用逆转录酶 - 聚合酶链 反应（RT-PCR）。 鼠骨纵向变化的测量 双能X射线吸收法（DXA或DEXA），A，A，A的矿物密度，A 我们为该程序开发的技术用于确定 衰老加速小鼠（SAM）和 它的杂种后代，随着年龄的年龄，腺切除术和 在Thee小鼠和其他菌株中的治疗以及前体的最佳时间 细胞培养和未钙化骨的组织形态计量学分析 标本。 Dexa取代的无创和精确的本质更多 传统的估计鼠骨幅度的方法，例如 肌宽度或皮质宽度形态测定法。 此核心也将获得，修复， 嵌入，截面，染色和量化骨骼组态图特征 老鼠和老鼠的轴向和阑尾骨骼满足 各种项目。 在导致此应用程序的初步工作中， 已经显而易见的是相邻骨骼的同时性能 RT-PCR的部分用于特定消息，并用细胞进行历史稳定 标记物，例如防trate抗性酸性磷酸酶（TRAP） 破骨细胞，非特异性酯酶（NSE）用于巨噬细胞或碱性 磷酸酶（ALP）的成骨细胞与四环素标记的骨 需要组织形态计量法来表征骨骼的不同子集和 骨髓细胞，其基因表达模式及其活性。 该核心在过去的体内进行了400多个鼠DEXA测量 六个月（周末上午8点共享使用临床密度计 到晚上10点或下午5点以后的工作日）并处理了85个人体骨头 活检和60多个鼠标本用于组织形态学检查 在过去的一年中。 骨实验室是帽子认可的设施 安全存档和存储组织和数据的能力。 骨 光密度计和组织形态计量结果将附加到一个 关系数据库，以及时返回到 每个项目。